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Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke.

Lin JB, Zheng CJ, Zhang X, Chen J, Liao WJ, Wan Q - Evid Based Complement Alternat Med (2015)

Bottom Line: TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites.TMP failed to affect the spine density of apical dendrites and the total dendritic length.Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

ABSTRACT
The 2,3,5,6-tetramethylpyrazine (TMP) has been widely used in the treatment of ischemic stroke by Chinese doctors. Here, we report the effects of TMP on functional recovery and dendritic plasticity after ischemic stroke. A classical model of middle cerebral artery occlusion (MCAO) was established in this study. The rats were assigned into 3 groups: sham group (sham operated rats treated with saline), model group (MCAO rats treated with saline) and TMP group (MCAO rats treated with 20 mg/kg/d TMP). The neurological function test of animals was evaluated using the modified neurological severity score (mNSS) at 3 d, 7 d, and 14 d after MCAO. Animals were euthanized for immunohistochemical labeling to measure MAP-2 levels in the peri-infarct area. Golgi-Cox staining was performed to test effect of TMP on dendritic plasticity at 14 d after MCAO. TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites. TMP failed to affect the spine density of apical dendrites and the total dendritic length. Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO. Thus, enhanced dendritic plasticity contributes to TMP-elicited functional recovery after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

Scatterplots present correlations analysis of mNSS and plasticity measured at 14 d after MCAO. (a) Scatterplots of mNSS and MAP-2 level. (b) Scatterplots of mNSS and total dendritic length. (c) Scatterplots of mNSS and spine density of basilar dendrites. (d) Scatterplots of mNSS and spine density of apical dendrites.
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fig10: Scatterplots present correlations analysis of mNSS and plasticity measured at 14 d after MCAO. (a) Scatterplots of mNSS and MAP-2 level. (b) Scatterplots of mNSS and total dendritic length. (c) Scatterplots of mNSS and spine density of basilar dendrites. (d) Scatterplots of mNSS and spine density of apical dendrites.

Mentions: The Spearman correlation coefficients test showed that there were significant negative correlations between mNSS and plasticity measured at 14 d after MCAO (mNSS and MAP-2: r = −0.619, P = 0.032; mNSS and total dendritic length: r = −0.640, P = 0.025; mNSS and spine density of basilar dendrites: r = −0.705, P = 0.010). But there was no significant correlation between mNSS and spine density of apical dendrites (r = −0.501, P = 0.097) (Figure 10).


Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke.

Lin JB, Zheng CJ, Zhang X, Chen J, Liao WJ, Wan Q - Evid Based Complement Alternat Med (2015)

Scatterplots present correlations analysis of mNSS and plasticity measured at 14 d after MCAO. (a) Scatterplots of mNSS and MAP-2 level. (b) Scatterplots of mNSS and total dendritic length. (c) Scatterplots of mNSS and spine density of basilar dendrites. (d) Scatterplots of mNSS and spine density of apical dendrites.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4563062&req=5

fig10: Scatterplots present correlations analysis of mNSS and plasticity measured at 14 d after MCAO. (a) Scatterplots of mNSS and MAP-2 level. (b) Scatterplots of mNSS and total dendritic length. (c) Scatterplots of mNSS and spine density of basilar dendrites. (d) Scatterplots of mNSS and spine density of apical dendrites.
Mentions: The Spearman correlation coefficients test showed that there were significant negative correlations between mNSS and plasticity measured at 14 d after MCAO (mNSS and MAP-2: r = −0.619, P = 0.032; mNSS and total dendritic length: r = −0.640, P = 0.025; mNSS and spine density of basilar dendrites: r = −0.705, P = 0.010). But there was no significant correlation between mNSS and spine density of apical dendrites (r = −0.501, P = 0.097) (Figure 10).

Bottom Line: TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites.TMP failed to affect the spine density of apical dendrites and the total dendritic length.Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

ABSTRACT
The 2,3,5,6-tetramethylpyrazine (TMP) has been widely used in the treatment of ischemic stroke by Chinese doctors. Here, we report the effects of TMP on functional recovery and dendritic plasticity after ischemic stroke. A classical model of middle cerebral artery occlusion (MCAO) was established in this study. The rats were assigned into 3 groups: sham group (sham operated rats treated with saline), model group (MCAO rats treated with saline) and TMP group (MCAO rats treated with 20 mg/kg/d TMP). The neurological function test of animals was evaluated using the modified neurological severity score (mNSS) at 3 d, 7 d, and 14 d after MCAO. Animals were euthanized for immunohistochemical labeling to measure MAP-2 levels in the peri-infarct area. Golgi-Cox staining was performed to test effect of TMP on dendritic plasticity at 14 d after MCAO. TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites. TMP failed to affect the spine density of apical dendrites and the total dendritic length. Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO. Thus, enhanced dendritic plasticity contributes to TMP-elicited functional recovery after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus