Limits...
Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke.

Lin JB, Zheng CJ, Zhang X, Chen J, Liao WJ, Wan Q - Evid Based Complement Alternat Med (2015)

Bottom Line: TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites.TMP failed to affect the spine density of apical dendrites and the total dendritic length.Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

ABSTRACT
The 2,3,5,6-tetramethylpyrazine (TMP) has been widely used in the treatment of ischemic stroke by Chinese doctors. Here, we report the effects of TMP on functional recovery and dendritic plasticity after ischemic stroke. A classical model of middle cerebral artery occlusion (MCAO) was established in this study. The rats were assigned into 3 groups: sham group (sham operated rats treated with saline), model group (MCAO rats treated with saline) and TMP group (MCAO rats treated with 20 mg/kg/d TMP). The neurological function test of animals was evaluated using the modified neurological severity score (mNSS) at 3 d, 7 d, and 14 d after MCAO. Animals were euthanized for immunohistochemical labeling to measure MAP-2 levels in the peri-infarct area. Golgi-Cox staining was performed to test effect of TMP on dendritic plasticity at 14 d after MCAO. TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites. TMP failed to affect the spine density of apical dendrites and the total dendritic length. Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO. Thus, enhanced dendritic plasticity contributes to TMP-elicited functional recovery after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

A representative photograph of TTC staining of MCAO rat.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4563062&req=5

fig4: A representative photograph of TTC staining of MCAO rat.

Mentions: Figure 4 shows a typical photograph of coronal sections of MCAO rat. The infarct region appeared white, and the normal tissue was red. Rats after MCAO exhibited obvious infarction which was located in cortex and striatum. The infarct volume was 38.42 ± 4.42%.


Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke.

Lin JB, Zheng CJ, Zhang X, Chen J, Liao WJ, Wan Q - Evid Based Complement Alternat Med (2015)

A representative photograph of TTC staining of MCAO rat.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4563062&req=5

fig4: A representative photograph of TTC staining of MCAO rat.
Mentions: Figure 4 shows a typical photograph of coronal sections of MCAO rat. The infarct region appeared white, and the normal tissue was red. Rats after MCAO exhibited obvious infarction which was located in cortex and striatum. The infarct volume was 38.42 ± 4.42%.

Bottom Line: TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites.TMP failed to affect the spine density of apical dendrites and the total dendritic length.Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

ABSTRACT
The 2,3,5,6-tetramethylpyrazine (TMP) has been widely used in the treatment of ischemic stroke by Chinese doctors. Here, we report the effects of TMP on functional recovery and dendritic plasticity after ischemic stroke. A classical model of middle cerebral artery occlusion (MCAO) was established in this study. The rats were assigned into 3 groups: sham group (sham operated rats treated with saline), model group (MCAO rats treated with saline) and TMP group (MCAO rats treated with 20 mg/kg/d TMP). The neurological function test of animals was evaluated using the modified neurological severity score (mNSS) at 3 d, 7 d, and 14 d after MCAO. Animals were euthanized for immunohistochemical labeling to measure MAP-2 levels in the peri-infarct area. Golgi-Cox staining was performed to test effect of TMP on dendritic plasticity at 14 d after MCAO. TMP significantly improved neurological function at 7 d and 14 d after ischemia, increased MAP-2 level at 14 d after ischemia, and enhanced spine density of basilar dendrites. TMP failed to affect the spine density of apical dendrites and the total dendritic length. Data analyses indicate that there was significant negative correlation between mNSS and plasticity measured at 14 d after MCAO. Thus, enhanced dendritic plasticity contributes to TMP-elicited functional recovery after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus