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Endogenous Asymmetric Dimethylarginine Pathway in High Altitude Adapted Yaks.

Mizuno S, Ishizaki T, Toga H, Sakai A, Isakova J, Taalaibekova E, Baiserkeev Z, Kojonazarov B, Aldashev A - Biomed Res Int (2015)

Bottom Line: We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks.Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations.The eNOS expression was significantly higher in yaks.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Kanazawa Medical University, Ishikawa 920-0293, Japan.

ABSTRACT
Hypoxia-induced and high altitude pulmonary hypertension are a major problem in the mountain areas of the world. The asymmetric methylarginines (ADMA) inhibit nitric oxide (NO) synthesis by competing with L-arginine, and high levels of plasma ADMA predict adverse outcomes in pulmonary hypertension. However, little is known about the regulation of the ADMA-NO pathway in animals adapted to high altitudes. We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks. Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations. The eNOS expression was significantly higher in yaks. These results indicate that augmented endogenous NO activity in yaks through the ADMA-DDAH pathway and eNOS upregulation account for the low pulmonary vascular tone observed in high altitude adapted yaks.

No MeSH data available.


Related in: MedlinePlus

DDAH activity in lungs from yaks and bovines. The bar graph shows DDAH I activity in the lungs from yaks and bovines. The DDAH activity is significantly increased in lungs from yaks compared with those from bovines. Data are expressed as mean ± SD (n = 6). ∗P < 0.05 versus bovine.
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fig3: DDAH activity in lungs from yaks and bovines. The bar graph shows DDAH I activity in the lungs from yaks and bovines. The DDAH activity is significantly increased in lungs from yaks compared with those from bovines. Data are expressed as mean ± SD (n = 6). ∗P < 0.05 versus bovine.

Mentions: The protein DDAH expression and the DDAH enzyme activity in the lungs from yaks were significantly higher, roughly twice, as high when compared with the bovine samples (Figure 3).


Endogenous Asymmetric Dimethylarginine Pathway in High Altitude Adapted Yaks.

Mizuno S, Ishizaki T, Toga H, Sakai A, Isakova J, Taalaibekova E, Baiserkeev Z, Kojonazarov B, Aldashev A - Biomed Res Int (2015)

DDAH activity in lungs from yaks and bovines. The bar graph shows DDAH I activity in the lungs from yaks and bovines. The DDAH activity is significantly increased in lungs from yaks compared with those from bovines. Data are expressed as mean ± SD (n = 6). ∗P < 0.05 versus bovine.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563057&req=5

fig3: DDAH activity in lungs from yaks and bovines. The bar graph shows DDAH I activity in the lungs from yaks and bovines. The DDAH activity is significantly increased in lungs from yaks compared with those from bovines. Data are expressed as mean ± SD (n = 6). ∗P < 0.05 versus bovine.
Mentions: The protein DDAH expression and the DDAH enzyme activity in the lungs from yaks were significantly higher, roughly twice, as high when compared with the bovine samples (Figure 3).

Bottom Line: We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks.Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations.The eNOS expression was significantly higher in yaks.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Kanazawa Medical University, Ishikawa 920-0293, Japan.

ABSTRACT
Hypoxia-induced and high altitude pulmonary hypertension are a major problem in the mountain areas of the world. The asymmetric methylarginines (ADMA) inhibit nitric oxide (NO) synthesis by competing with L-arginine, and high levels of plasma ADMA predict adverse outcomes in pulmonary hypertension. However, little is known about the regulation of the ADMA-NO pathway in animals adapted to high altitudes. We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks. Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations. The eNOS expression was significantly higher in yaks. These results indicate that augmented endogenous NO activity in yaks through the ADMA-DDAH pathway and eNOS upregulation account for the low pulmonary vascular tone observed in high altitude adapted yaks.

No MeSH data available.


Related in: MedlinePlus