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Endogenous Asymmetric Dimethylarginine Pathway in High Altitude Adapted Yaks.

Mizuno S, Ishizaki T, Toga H, Sakai A, Isakova J, Taalaibekova E, Baiserkeev Z, Kojonazarov B, Aldashev A - Biomed Res Int (2015)

Bottom Line: We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks.Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations.The eNOS expression was significantly higher in yaks.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Kanazawa Medical University, Ishikawa 920-0293, Japan.

ABSTRACT
Hypoxia-induced and high altitude pulmonary hypertension are a major problem in the mountain areas of the world. The asymmetric methylarginines (ADMA) inhibit nitric oxide (NO) synthesis by competing with L-arginine, and high levels of plasma ADMA predict adverse outcomes in pulmonary hypertension. However, little is known about the regulation of the ADMA-NO pathway in animals adapted to high altitudes. We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks. Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations. The eNOS expression was significantly higher in yaks. These results indicate that augmented endogenous NO activity in yaks through the ADMA-DDAH pathway and eNOS upregulation account for the low pulmonary vascular tone observed in high altitude adapted yaks.

No MeSH data available.


Related in: MedlinePlus

Nitrates, ADMA, and endothelin-1 (ET-1) concentrations of plasma from yaks and bovines. Nitrate concentration, measured by Griess method, showed no significant differences between yaks (n = 12) and bovines (n = 6) (a). ADMA concentration, measured by ELISA, was significantly lower in yaks (n = 19) than bovines (n = 11) (b), and ET-1 concentration was significantly higher in yaks (n = 13) than bovines (n = 10) (c). Data are expressed as mean ± SD. ∗P < 0.05 versus bovine.
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fig1: Nitrates, ADMA, and endothelin-1 (ET-1) concentrations of plasma from yaks and bovines. Nitrate concentration, measured by Griess method, showed no significant differences between yaks (n = 12) and bovines (n = 6) (a). ADMA concentration, measured by ELISA, was significantly lower in yaks (n = 19) than bovines (n = 11) (b), and ET-1 concentration was significantly higher in yaks (n = 13) than bovines (n = 10) (c). Data are expressed as mean ± SD. ∗P < 0.05 versus bovine.

Mentions: Although the average nitrate concentration is slightly higher in yaks when compared to that in the bovine, there were no significant differences in serum nitrates concentrations between bovines and yaks. The serum levels of ET-1 in yaks were significantly increased compared with that from bovines, and the serum ADMA levels were significantly decreased compared with that from bovines (Figures 1(a), 1(b) and 1(c)).


Endogenous Asymmetric Dimethylarginine Pathway in High Altitude Adapted Yaks.

Mizuno S, Ishizaki T, Toga H, Sakai A, Isakova J, Taalaibekova E, Baiserkeev Z, Kojonazarov B, Aldashev A - Biomed Res Int (2015)

Nitrates, ADMA, and endothelin-1 (ET-1) concentrations of plasma from yaks and bovines. Nitrate concentration, measured by Griess method, showed no significant differences between yaks (n = 12) and bovines (n = 6) (a). ADMA concentration, measured by ELISA, was significantly lower in yaks (n = 19) than bovines (n = 11) (b), and ET-1 concentration was significantly higher in yaks (n = 13) than bovines (n = 10) (c). Data are expressed as mean ± SD. ∗P < 0.05 versus bovine.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563057&req=5

fig1: Nitrates, ADMA, and endothelin-1 (ET-1) concentrations of plasma from yaks and bovines. Nitrate concentration, measured by Griess method, showed no significant differences between yaks (n = 12) and bovines (n = 6) (a). ADMA concentration, measured by ELISA, was significantly lower in yaks (n = 19) than bovines (n = 11) (b), and ET-1 concentration was significantly higher in yaks (n = 13) than bovines (n = 10) (c). Data are expressed as mean ± SD. ∗P < 0.05 versus bovine.
Mentions: Although the average nitrate concentration is slightly higher in yaks when compared to that in the bovine, there were no significant differences in serum nitrates concentrations between bovines and yaks. The serum levels of ET-1 in yaks were significantly increased compared with that from bovines, and the serum ADMA levels were significantly decreased compared with that from bovines (Figures 1(a), 1(b) and 1(c)).

Bottom Line: We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks.Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations.The eNOS expression was significantly higher in yaks.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Kanazawa Medical University, Ishikawa 920-0293, Japan.

ABSTRACT
Hypoxia-induced and high altitude pulmonary hypertension are a major problem in the mountain areas of the world. The asymmetric methylarginines (ADMA) inhibit nitric oxide (NO) synthesis by competing with L-arginine, and high levels of plasma ADMA predict adverse outcomes in pulmonary hypertension. However, little is known about the regulation of the ADMA-NO pathway in animals adapted to high altitudes. We measured the plasma ADMA concentration, endothelial NO synthase (eNOS), dimethylarginine dimethylaminohydrolases (DDAH) protein expression, and DDAH activities in the lungs from yaks. Although the yaks are hypoxemic, cardiac function and pulmonary arterial pressures are almost normal, and we found decreased DDAH expression and activity in association with reduced plasma ADMA concentrations. The eNOS expression was significantly higher in yaks. These results indicate that augmented endogenous NO activity in yaks through the ADMA-DDAH pathway and eNOS upregulation account for the low pulmonary vascular tone observed in high altitude adapted yaks.

No MeSH data available.


Related in: MedlinePlus