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Effects of nutritional supplementation with l-arginine on repair of injuries due to muscle strain: experimental study on rats.

Couto LI, Wuicik WL, Kuhn I, Capriotti JR, Repka JC - Rev Bras Ortop (2015)

Bottom Line: The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Hospital Angelina Caron, Campina Grande do Sul, PR, Brazil.

ABSTRACT

Objective: To evaluate the influence of oral supplementation with arginine on regeneration of injuries due to straining of the anterior tibial muscle of rats.

Methods: Twenty-four Wistar rats of weight 492.5 ± 50.45 g were used. Injuries were induced through straining the anterior tibial muscles. The rats were separated into three groups of eight rats each. In the untreated group (UTG), after induction of injuries, the rats were observed for 24 h. In the simulation group (SG) and the arginine group (AG) respectively, the rats received isotonic saline solution and arginine solution via direct gavage, over a seven-day period. At the end of the period, blood samples were collected for serum evaluations of creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and C-reactive protein (CRP). The right and left anterior tibial muscles were resected for histopathological evaluations on the muscle injuries, investigating edema, hemorrhage and disorganization or morphometric alteration of the muscle fibers. The tissue repair was investigated in terms of proliferation of adipose tissue, angiogenesis and collagen fibers. The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.

Results: In the serum evaluations, the AG showed lower CK assay values and higher AST values. In the histopathological evaluation, the UTG presented edema and hemorrhage compatible with injuries due to strain; the SG presented edema and hemorrhage with proliferation of adipose tissue and collagen fibers; and the AG presented not only the findings of the SG but also, especially, intense angiogenesis.

Conclusion: Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.

No MeSH data available.


Related in: MedlinePlus

Graphs representing the means and standard deviations of the biochemical assays, comparing the three groups: UTG (untreated and subjected to muscle traction), SG (simulation) and AG (arginine).
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fig0010: Graphs representing the means and standard deviations of the biochemical assays, comparing the three groups: UTG (untreated and subjected to muscle traction), SG (simulation) and AG (arginine).

Mentions: In the biochemical evaluations, there was a difference between the groups with regard to the means from the assays on creatine kinase (p = 0.0014) and aspartate aminotransferase (p = 0.0150). Regarding the means from the assays on lactic dehydrogenase (p = 0.3331) and C-reactive protein (p = 0.1149), there was no difference between the groups (Fig. 2). It can be seen from detail A of Fig. 2 that the mean from the assay on creatine kinase in the UTG was significant greater than the means in the SG and AG, and that the mean in the AG was significantly lower than the mean in the SG. In detail C, it can be seen that there was a significant difference between UTG and SG, but not between SG and AG.


Effects of nutritional supplementation with l-arginine on repair of injuries due to muscle strain: experimental study on rats.

Couto LI, Wuicik WL, Kuhn I, Capriotti JR, Repka JC - Rev Bras Ortop (2015)

Graphs representing the means and standard deviations of the biochemical assays, comparing the three groups: UTG (untreated and subjected to muscle traction), SG (simulation) and AG (arginine).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563043&req=5

fig0010: Graphs representing the means and standard deviations of the biochemical assays, comparing the three groups: UTG (untreated and subjected to muscle traction), SG (simulation) and AG (arginine).
Mentions: In the biochemical evaluations, there was a difference between the groups with regard to the means from the assays on creatine kinase (p = 0.0014) and aspartate aminotransferase (p = 0.0150). Regarding the means from the assays on lactic dehydrogenase (p = 0.3331) and C-reactive protein (p = 0.1149), there was no difference between the groups (Fig. 2). It can be seen from detail A of Fig. 2 that the mean from the assay on creatine kinase in the UTG was significant greater than the means in the SG and AG, and that the mean in the AG was significantly lower than the mean in the SG. In detail C, it can be seen that there was a significant difference between UTG and SG, but not between SG and AG.

Bottom Line: The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Hospital Angelina Caron, Campina Grande do Sul, PR, Brazil.

ABSTRACT

Objective: To evaluate the influence of oral supplementation with arginine on regeneration of injuries due to straining of the anterior tibial muscle of rats.

Methods: Twenty-four Wistar rats of weight 492.5 ± 50.45 g were used. Injuries were induced through straining the anterior tibial muscles. The rats were separated into three groups of eight rats each. In the untreated group (UTG), after induction of injuries, the rats were observed for 24 h. In the simulation group (SG) and the arginine group (AG) respectively, the rats received isotonic saline solution and arginine solution via direct gavage, over a seven-day period. At the end of the period, blood samples were collected for serum evaluations of creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and C-reactive protein (CRP). The right and left anterior tibial muscles were resected for histopathological evaluations on the muscle injuries, investigating edema, hemorrhage and disorganization or morphometric alteration of the muscle fibers. The tissue repair was investigated in terms of proliferation of adipose tissue, angiogenesis and collagen fibers. The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.

Results: In the serum evaluations, the AG showed lower CK assay values and higher AST values. In the histopathological evaluation, the UTG presented edema and hemorrhage compatible with injuries due to strain; the SG presented edema and hemorrhage with proliferation of adipose tissue and collagen fibers; and the AG presented not only the findings of the SG but also, especially, intense angiogenesis.

Conclusion: Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.

No MeSH data available.


Related in: MedlinePlus