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Effects of nutritional supplementation with l-arginine on repair of injuries due to muscle strain: experimental study on rats.

Couto LI, Wuicik WL, Kuhn I, Capriotti JR, Repka JC - Rev Bras Ortop (2015)

Bottom Line: The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Hospital Angelina Caron, Campina Grande do Sul, PR, Brazil.

ABSTRACT

Objective: To evaluate the influence of oral supplementation with arginine on regeneration of injuries due to straining of the anterior tibial muscle of rats.

Methods: Twenty-four Wistar rats of weight 492.5 ± 50.45 g were used. Injuries were induced through straining the anterior tibial muscles. The rats were separated into three groups of eight rats each. In the untreated group (UTG), after induction of injuries, the rats were observed for 24 h. In the simulation group (SG) and the arginine group (AG) respectively, the rats received isotonic saline solution and arginine solution via direct gavage, over a seven-day period. At the end of the period, blood samples were collected for serum evaluations of creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and C-reactive protein (CRP). The right and left anterior tibial muscles were resected for histopathological evaluations on the muscle injuries, investigating edema, hemorrhage and disorganization or morphometric alteration of the muscle fibers. The tissue repair was investigated in terms of proliferation of adipose tissue, angiogenesis and collagen fibers. The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.

Results: In the serum evaluations, the AG showed lower CK assay values and higher AST values. In the histopathological evaluation, the UTG presented edema and hemorrhage compatible with injuries due to strain; the SG presented edema and hemorrhage with proliferation of adipose tissue and collagen fibers; and the AG presented not only the findings of the SG but also, especially, intense angiogenesis.

Conclusion: Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.

No MeSH data available.


Related in: MedlinePlus

Details of the system used for inducing strain in the anterior tibial muscles of rats under anesthesia: (*) right hind legs under traction; (**) pulleys enabling suspension of plastic bags containing water.
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fig0005: Details of the system used for inducing strain in the anterior tibial muscles of rats under anesthesia: (*) right hind legs under traction; (**) pulleys enabling suspension of plastic bags containing water.

Mentions: It was standardized that traction would only be performed on the right hind leg. The apparatus used had previously been described19 and had been specially constructed for the purpose of inducing muscle injury through straining, as demonstrated in Fig. 1. In this, groups of five rats under anesthesia were individually fixed in dorsal decubitus to the apparatus using adhesive tape, with the dorsum of the paw of the right hind leg attached to a string using adhesive tape. This string passed over a pulley and a freely hanging plastic bag containing a volume of water corresponding to 150% of the weight of the respective rat was attached to the other end of the string. In this manner, plantar flexion was performed for 45 min, which caused an injury due to straining of the anterior tibial muscle. After induction of the muscle injury, the rats were transferred to a specific environment. The UTG rats were kept there for 24 h and the SG and AG rats for seven days. After these maintenance periods and the nutritional supplementation described earlier, blood collection was performed, followed by resection of the anterior tibial muscles of both of the hind legs, for serum biochemical and histopathological evaluations.


Effects of nutritional supplementation with l-arginine on repair of injuries due to muscle strain: experimental study on rats.

Couto LI, Wuicik WL, Kuhn I, Capriotti JR, Repka JC - Rev Bras Ortop (2015)

Details of the system used for inducing strain in the anterior tibial muscles of rats under anesthesia: (*) right hind legs under traction; (**) pulleys enabling suspension of plastic bags containing water.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563043&req=5

fig0005: Details of the system used for inducing strain in the anterior tibial muscles of rats under anesthesia: (*) right hind legs under traction; (**) pulleys enabling suspension of plastic bags containing water.
Mentions: It was standardized that traction would only be performed on the right hind leg. The apparatus used had previously been described19 and had been specially constructed for the purpose of inducing muscle injury through straining, as demonstrated in Fig. 1. In this, groups of five rats under anesthesia were individually fixed in dorsal decubitus to the apparatus using adhesive tape, with the dorsum of the paw of the right hind leg attached to a string using adhesive tape. This string passed over a pulley and a freely hanging plastic bag containing a volume of water corresponding to 150% of the weight of the respective rat was attached to the other end of the string. In this manner, plantar flexion was performed for 45 min, which caused an injury due to straining of the anterior tibial muscle. After induction of the muscle injury, the rats were transferred to a specific environment. The UTG rats were kept there for 24 h and the SG and AG rats for seven days. After these maintenance periods and the nutritional supplementation described earlier, blood collection was performed, followed by resection of the anterior tibial muscles of both of the hind legs, for serum biochemical and histopathological evaluations.

Bottom Line: The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Hospital Angelina Caron, Campina Grande do Sul, PR, Brazil.

ABSTRACT

Objective: To evaluate the influence of oral supplementation with arginine on regeneration of injuries due to straining of the anterior tibial muscle of rats.

Methods: Twenty-four Wistar rats of weight 492.5 ± 50.45 g were used. Injuries were induced through straining the anterior tibial muscles. The rats were separated into three groups of eight rats each. In the untreated group (UTG), after induction of injuries, the rats were observed for 24 h. In the simulation group (SG) and the arginine group (AG) respectively, the rats received isotonic saline solution and arginine solution via direct gavage, over a seven-day period. At the end of the period, blood samples were collected for serum evaluations of creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and C-reactive protein (CRP). The right and left anterior tibial muscles were resected for histopathological evaluations on the muscle injuries, investigating edema, hemorrhage and disorganization or morphometric alteration of the muscle fibers. The tissue repair was investigated in terms of proliferation of adipose tissue, angiogenesis and collagen fibers. The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.

Results: In the serum evaluations, the AG showed lower CK assay values and higher AST values. In the histopathological evaluation, the UTG presented edema and hemorrhage compatible with injuries due to strain; the SG presented edema and hemorrhage with proliferation of adipose tissue and collagen fibers; and the AG presented not only the findings of the SG but also, especially, intense angiogenesis.

Conclusion: Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.

No MeSH data available.


Related in: MedlinePlus