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Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus

Correlation between cytokine concentrations in supernatant of three-dimensionally cultured normal tissues and the WST-8 assay values of cultured tissues. Correlation was evaluated by Pearson correlation coefficient test and Spearman correlation coefficient test (n = 8). Concentrations for IL-10 (B) in the culture supernatant was significantly correlated with the WST-8 assay values of the tissues (P = 0.0458 by Spearman); however, there was no significant correlation between normal tissue growth and IL-6 concentration (A).
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f0025: Correlation between cytokine concentrations in supernatant of three-dimensionally cultured normal tissues and the WST-8 assay values of cultured tissues. Correlation was evaluated by Pearson correlation coefficient test and Spearman correlation coefficient test (n = 8). Concentrations for IL-10 (B) in the culture supernatant was significantly correlated with the WST-8 assay values of the tissues (P = 0.0458 by Spearman); however, there was no significant correlation between normal tissue growth and IL-6 concentration (A).

Mentions: The growth abilities of cancer tissues as assessed by WST-8 assay significantly correlated positively with concentrations of IFN-γ (A; P = 0.0031 by Pearson), IL-6 (D; P = 0.0274 by Pearson and P = 0.0009 by Spearman), and IL-10 (E; P = 0.0158 by Pearson and P = 0.0003 by Spearman), whereas those of TNF-α (B), IL-2 (C), and IL-17A (F) did not (Figure 4). The growth abilities of normal mammary tissues were significantly correlated positively with concentrations of IL-10 (P = 0.0458) (Figure 5B); however, there was no significant correlation between normal tissue growth and IL-6 concentration (Figure 5A).


Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Correlation between cytokine concentrations in supernatant of three-dimensionally cultured normal tissues and the WST-8 assay values of cultured tissues. Correlation was evaluated by Pearson correlation coefficient test and Spearman correlation coefficient test (n = 8). Concentrations for IL-10 (B) in the culture supernatant was significantly correlated with the WST-8 assay values of the tissues (P = 0.0458 by Spearman); however, there was no significant correlation between normal tissue growth and IL-6 concentration (A).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562984&req=5

f0025: Correlation between cytokine concentrations in supernatant of three-dimensionally cultured normal tissues and the WST-8 assay values of cultured tissues. Correlation was evaluated by Pearson correlation coefficient test and Spearman correlation coefficient test (n = 8). Concentrations for IL-10 (B) in the culture supernatant was significantly correlated with the WST-8 assay values of the tissues (P = 0.0458 by Spearman); however, there was no significant correlation between normal tissue growth and IL-6 concentration (A).
Mentions: The growth abilities of cancer tissues as assessed by WST-8 assay significantly correlated positively with concentrations of IFN-γ (A; P = 0.0031 by Pearson), IL-6 (D; P = 0.0274 by Pearson and P = 0.0009 by Spearman), and IL-10 (E; P = 0.0158 by Pearson and P = 0.0003 by Spearman), whereas those of TNF-α (B), IL-2 (C), and IL-17A (F) did not (Figure 4). The growth abilities of normal mammary tissues were significantly correlated positively with concentrations of IL-10 (P = 0.0458) (Figure 5B); however, there was no significant correlation between normal tissue growth and IL-6 concentration (Figure 5A).

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus