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Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus

Comparison of each cytokine concentration in supernatant of three-dimensionally cultured tissues obtained from patients with and without chemotherapy. IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A in the supernatant of three-dimensionally cultured tissues obtained from patients with (n = 6) or without (n = 15) chemotherapy were compared. There was no difference in IFN-γ (A; P = 0.0662), TNF-α (B; P = 0.1612), IL-2 (C; P = 0.4254), IL-6 (D; P = 0.1106), IL-10 (E; P = 0.0674), and IL-17A (F; P = 0.7086) concentrations between these patient subgroups, as analyzed by Mann-Whitney U test.
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f0015: Comparison of each cytokine concentration in supernatant of three-dimensionally cultured tissues obtained from patients with and without chemotherapy. IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A in the supernatant of three-dimensionally cultured tissues obtained from patients with (n = 6) or without (n = 15) chemotherapy were compared. There was no difference in IFN-γ (A; P = 0.0662), TNF-α (B; P = 0.1612), IL-2 (C; P = 0.4254), IL-6 (D; P = 0.1106), IL-10 (E; P = 0.0674), and IL-17A (F; P = 0.7086) concentrations between these patient subgroups, as analyzed by Mann-Whitney U test.

Mentions: The concentrations of IFN-γ and IL-10 in the supernatant of cancer tissues obtained from the patients who underwent chemotherapy (n = 6) tended to be higher than those in the supernatant of cancer tissues obtained from the patients without chemotherapy (n = 15), although the differences were not statistically significant (Figure 3). There was no difference in IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A concentrations between these patient subgroups (Figure 3).


Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Comparison of each cytokine concentration in supernatant of three-dimensionally cultured tissues obtained from patients with and without chemotherapy. IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A in the supernatant of three-dimensionally cultured tissues obtained from patients with (n = 6) or without (n = 15) chemotherapy were compared. There was no difference in IFN-γ (A; P = 0.0662), TNF-α (B; P = 0.1612), IL-2 (C; P = 0.4254), IL-6 (D; P = 0.1106), IL-10 (E; P = 0.0674), and IL-17A (F; P = 0.7086) concentrations between these patient subgroups, as analyzed by Mann-Whitney U test.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562984&req=5

f0015: Comparison of each cytokine concentration in supernatant of three-dimensionally cultured tissues obtained from patients with and without chemotherapy. IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A in the supernatant of three-dimensionally cultured tissues obtained from patients with (n = 6) or without (n = 15) chemotherapy were compared. There was no difference in IFN-γ (A; P = 0.0662), TNF-α (B; P = 0.1612), IL-2 (C; P = 0.4254), IL-6 (D; P = 0.1106), IL-10 (E; P = 0.0674), and IL-17A (F; P = 0.7086) concentrations between these patient subgroups, as analyzed by Mann-Whitney U test.
Mentions: The concentrations of IFN-γ and IL-10 in the supernatant of cancer tissues obtained from the patients who underwent chemotherapy (n = 6) tended to be higher than those in the supernatant of cancer tissues obtained from the patients without chemotherapy (n = 15), although the differences were not statistically significant (Figure 3). There was no difference in IFN-γ, TNF-α, IL-2, IL-6, IL-10, and IL-17A concentrations between these patient subgroups (Figure 3).

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus