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Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus

Comparison of cytokine concentrations in supernatant of three-dimensionally cultured breast cancer and normal tissues. Breast cancer tissues (cancer tissues) or normal tissues of breast cancer patients (normal tissues) were cultured for 6 days in TGP gel, respectively, and the cytokine concentrations in supernatant were determined by CBA assay procedures. Twenty-one cancer tissues and eight normal tissues were examined. The mean ± SD concentrations were indicated. The cytokine concentrations did not significantly differ between cancer tissues and normal tissues. The frequencies of the expression of IFN-γ were significantly different between the cancer tissues and the normal tissues as analyzed by χ2 test (P = 0.0052). ND (not detected): The cytokine concentrations were under each detection limit.
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f0010: Comparison of cytokine concentrations in supernatant of three-dimensionally cultured breast cancer and normal tissues. Breast cancer tissues (cancer tissues) or normal tissues of breast cancer patients (normal tissues) were cultured for 6 days in TGP gel, respectively, and the cytokine concentrations in supernatant were determined by CBA assay procedures. Twenty-one cancer tissues and eight normal tissues were examined. The mean ± SD concentrations were indicated. The cytokine concentrations did not significantly differ between cancer tissues and normal tissues. The frequencies of the expression of IFN-γ were significantly different between the cancer tissues and the normal tissues as analyzed by χ2 test (P = 0.0052). ND (not detected): The cytokine concentrations were under each detection limit.

Mentions: We measured the concentrations of IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17A secreted in the supernatant of three-dimensionally cultured breast cancer tissues (n = 21) and normal mammary tissues (n = 8). Both of the cancer and the normal mammary tissues secreted IL-6 and IL-10 in the supernatant (Figure 2). However, IFN-γ, TNF-α, IL-2, and IL-17A were only detected in the supernatant of cancer tissues (Figure 2). IL-4 was not detected in each culture supernatant (Figure 2). There were no significant differences in IL-6 and IL-10 concentrations between cancer and normal tissues. The frequency for the excretion of IFN-γ into the culture supernatant of breast cancer tissues was statistically higher than that of the normal mammary tissues (P = 0.0052) (Table 2). Thus, IFN-γ is considered to be specific to cancer tissues.


Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Comparison of cytokine concentrations in supernatant of three-dimensionally cultured breast cancer and normal tissues. Breast cancer tissues (cancer tissues) or normal tissues of breast cancer patients (normal tissues) were cultured for 6 days in TGP gel, respectively, and the cytokine concentrations in supernatant were determined by CBA assay procedures. Twenty-one cancer tissues and eight normal tissues were examined. The mean ± SD concentrations were indicated. The cytokine concentrations did not significantly differ between cancer tissues and normal tissues. The frequencies of the expression of IFN-γ were significantly different between the cancer tissues and the normal tissues as analyzed by χ2 test (P = 0.0052). ND (not detected): The cytokine concentrations were under each detection limit.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562984&req=5

f0010: Comparison of cytokine concentrations in supernatant of three-dimensionally cultured breast cancer and normal tissues. Breast cancer tissues (cancer tissues) or normal tissues of breast cancer patients (normal tissues) were cultured for 6 days in TGP gel, respectively, and the cytokine concentrations in supernatant were determined by CBA assay procedures. Twenty-one cancer tissues and eight normal tissues were examined. The mean ± SD concentrations were indicated. The cytokine concentrations did not significantly differ between cancer tissues and normal tissues. The frequencies of the expression of IFN-γ were significantly different between the cancer tissues and the normal tissues as analyzed by χ2 test (P = 0.0052). ND (not detected): The cytokine concentrations were under each detection limit.
Mentions: We measured the concentrations of IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17A secreted in the supernatant of three-dimensionally cultured breast cancer tissues (n = 21) and normal mammary tissues (n = 8). Both of the cancer and the normal mammary tissues secreted IL-6 and IL-10 in the supernatant (Figure 2). However, IFN-γ, TNF-α, IL-2, and IL-17A were only detected in the supernatant of cancer tissues (Figure 2). IL-4 was not detected in each culture supernatant (Figure 2). There were no significant differences in IL-6 and IL-10 concentrations between cancer and normal tissues. The frequency for the excretion of IFN-γ into the culture supernatant of breast cancer tissues was statistically higher than that of the normal mammary tissues (P = 0.0052) (Table 2). Thus, IFN-γ is considered to be specific to cancer tissues.

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus