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Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus

Growth abilities of three-dimensionally cultured breast cancer tissues. The growth abilities of the tissues three-dimensionally cultured in TGP gel were assessed by WST-8 assay (absorbance at 450 nm; see Materials and Methods) after the culture. (A) Comparison of the growth abilities between breast cancer tissues (n = 21) and normal breast tissues (n = 9). No significant difference was observed as analyzed by Wilcoxon signed rank test (P = 0.3125). (B) Comparison between cancer tissues from patients treated by chemotherapy (n = 6) and those from patients who were not treated by chemotherapy (n = 15). No significant difference was observed as analyzed by Mann-Whitney test (P = 0.1291).
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f0005: Growth abilities of three-dimensionally cultured breast cancer tissues. The growth abilities of the tissues three-dimensionally cultured in TGP gel were assessed by WST-8 assay (absorbance at 450 nm; see Materials and Methods) after the culture. (A) Comparison of the growth abilities between breast cancer tissues (n = 21) and normal breast tissues (n = 9). No significant difference was observed as analyzed by Wilcoxon signed rank test (P = 0.3125). (B) Comparison between cancer tissues from patients treated by chemotherapy (n = 6) and those from patients who were not treated by chemotherapy (n = 15). No significant difference was observed as analyzed by Mann-Whitney test (P = 0.1291).

Mentions: Growth abilities of three-dimensionally cultured breast cancer tissues (n = 21) and normal mammary tissues (n = 9) were analyzed by WST assay. Whereas cancer tissues tended to show higher growth potential than normal tissues, there was no significant difference (P = 0.3125) (Figure 1A). Growth abilities of breast cancer tissues resected from patients under chemotherapy (n = 6) seemed to be higher than those from the patients without chemotherapy (n = 15), but there was no significant difference (P = 0.1291) (Figure 1B).


Characterization and Clinical Implication of Th1/Th2/Th17 Cytokines Produced from Three-Dimensionally Cultured Tumor Tissues Resected from Breast Cancer Patients.

Kiyomi A, Makita M, Ozeki T, Li N, Satomura A, Tanaka S, Onda K, Sugiyama K, Iwase T, Hirano T - Transl Oncol (2015)

Growth abilities of three-dimensionally cultured breast cancer tissues. The growth abilities of the tissues three-dimensionally cultured in TGP gel were assessed by WST-8 assay (absorbance at 450 nm; see Materials and Methods) after the culture. (A) Comparison of the growth abilities between breast cancer tissues (n = 21) and normal breast tissues (n = 9). No significant difference was observed as analyzed by Wilcoxon signed rank test (P = 0.3125). (B) Comparison between cancer tissues from patients treated by chemotherapy (n = 6) and those from patients who were not treated by chemotherapy (n = 15). No significant difference was observed as analyzed by Mann-Whitney test (P = 0.1291).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562984&req=5

f0005: Growth abilities of three-dimensionally cultured breast cancer tissues. The growth abilities of the tissues three-dimensionally cultured in TGP gel were assessed by WST-8 assay (absorbance at 450 nm; see Materials and Methods) after the culture. (A) Comparison of the growth abilities between breast cancer tissues (n = 21) and normal breast tissues (n = 9). No significant difference was observed as analyzed by Wilcoxon signed rank test (P = 0.3125). (B) Comparison between cancer tissues from patients treated by chemotherapy (n = 6) and those from patients who were not treated by chemotherapy (n = 15). No significant difference was observed as analyzed by Mann-Whitney test (P = 0.1291).
Mentions: Growth abilities of three-dimensionally cultured breast cancer tissues (n = 21) and normal mammary tissues (n = 9) were analyzed by WST assay. Whereas cancer tissues tended to show higher growth potential than normal tissues, there was no significant difference (P = 0.3125) (Figure 1A). Growth abilities of breast cancer tissues resected from patients under chemotherapy (n = 6) seemed to be higher than those from the patients without chemotherapy (n = 15), but there was no significant difference (P = 0.1291) (Figure 1B).

Bottom Line: Cancer tissues of stage ≥IIB patients expressed significantly higher TNF-α levels as compared with those of patients with stage <IIB (P=0.0096).The tumor tissues resected from breast cancer patients can grow in the three-dimensional thermoreversible gelatin polymer culture system and produce Th1/Th2/Th17 cytokines.TNF-α is suggested to be a biomarker for the cancer stage.

View Article: PubMed Central - PubMed

Affiliation: Tokyo University of Pharmacy and Life Sciences, Department of Clinical Pharmacology, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: y064076@toyaku.ac.jp.

No MeSH data available.


Related in: MedlinePlus