Limits...
Enhancement of the Immunostimulatory Functions of Ex Vivo-Generated Dendritic Cells from Early-Stage Colon Cancer Patients by Consecutive Exposure to Low Doses of Sequential-Kinetic-Activated IL-4 and IL-12. A Preliminary Study.

Radice E, Bellone G, Miranda V - Transl Oncol (2015)

Bottom Line: Because of their potent immunoregulatory capacities, DCs have been exploited in anticancer vaccination, with limited success thus far.No single agent enhanced the compromised allostimulatory activity of MoDCs from colon cancer patients, unlike healthy donors.However, MoDCs from nonmetastatic colon cancer patients, after sequential exposure to SKA-IL-4 (48 hours) and SKA-IL-12 (24 hours), displayed increased T-cell stimulatory capacity by MLR and acquired driving T-helper 1 polarization activity, although less markedly than the effects induced by recombinant human cytokines or found in normal subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Sciences, Corso Dogliotti 14, 10126 Turin, University of Turin, Italy. Electronic address: elisabetta.radice@unito.it.

No MeSH data available.


Related in: MedlinePlus

(A) MoDC allostimulatory activity of colon carcinoma patients (n = 16) versus healthy donors (n = 12). (B) MoDC allostimulatory activity of nonmetastatic (n = 9) versus metastatic (n = 7) colon carcinoma patients. MoDCs, generated by culturing PB CD14+ cells from tumor and normal subjects in the presence of rhGM-CSF and rhIL-4 for 6 days, were incubated with 1 × 105 allogeneic naïve CD4+ T cells at 1:40, 1:20, and 1:10 ratios for 5 days followed by a 6-hour pulse of 3H-TdR. Results are expressed as mean ± SE cpm of triplicate co-cultures. Statistical significance was determined using one-way ANOVA.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4562983&req=5

f0005: (A) MoDC allostimulatory activity of colon carcinoma patients (n = 16) versus healthy donors (n = 12). (B) MoDC allostimulatory activity of nonmetastatic (n = 9) versus metastatic (n = 7) colon carcinoma patients. MoDCs, generated by culturing PB CD14+ cells from tumor and normal subjects in the presence of rhGM-CSF and rhIL-4 for 6 days, were incubated with 1 × 105 allogeneic naïve CD4+ T cells at 1:40, 1:20, and 1:10 ratios for 5 days followed by a 6-hour pulse of 3H-TdR. Results are expressed as mean ± SE cpm of triplicate co-cultures. Statistical significance was determined using one-way ANOVA.

Mentions: It was first assessed whether MoDC cells from colon carcinoma patients (n = 16) retained stimulatory aptitude comparable to that of healthy-donor counterparts (n = 12). Notably, as shown in Figure 1A, CD4+-naïve T lymphocytes after incubation with allogeneic MoDCs from colon carcinoma patients exhibited a significantly lower proliferative response (indicated by 3H-TdR uptake) (almost 10 times less) than did those stimulated with normal MoDCs (P ≤ .001). Colon carcinoma patients thus demonstrated a marked defect in DC functional activity.


Enhancement of the Immunostimulatory Functions of Ex Vivo-Generated Dendritic Cells from Early-Stage Colon Cancer Patients by Consecutive Exposure to Low Doses of Sequential-Kinetic-Activated IL-4 and IL-12. A Preliminary Study.

Radice E, Bellone G, Miranda V - Transl Oncol (2015)

(A) MoDC allostimulatory activity of colon carcinoma patients (n = 16) versus healthy donors (n = 12). (B) MoDC allostimulatory activity of nonmetastatic (n = 9) versus metastatic (n = 7) colon carcinoma patients. MoDCs, generated by culturing PB CD14+ cells from tumor and normal subjects in the presence of rhGM-CSF and rhIL-4 for 6 days, were incubated with 1 × 105 allogeneic naïve CD4+ T cells at 1:40, 1:20, and 1:10 ratios for 5 days followed by a 6-hour pulse of 3H-TdR. Results are expressed as mean ± SE cpm of triplicate co-cultures. Statistical significance was determined using one-way ANOVA.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562983&req=5

f0005: (A) MoDC allostimulatory activity of colon carcinoma patients (n = 16) versus healthy donors (n = 12). (B) MoDC allostimulatory activity of nonmetastatic (n = 9) versus metastatic (n = 7) colon carcinoma patients. MoDCs, generated by culturing PB CD14+ cells from tumor and normal subjects in the presence of rhGM-CSF and rhIL-4 for 6 days, were incubated with 1 × 105 allogeneic naïve CD4+ T cells at 1:40, 1:20, and 1:10 ratios for 5 days followed by a 6-hour pulse of 3H-TdR. Results are expressed as mean ± SE cpm of triplicate co-cultures. Statistical significance was determined using one-way ANOVA.
Mentions: It was first assessed whether MoDC cells from colon carcinoma patients (n = 16) retained stimulatory aptitude comparable to that of healthy-donor counterparts (n = 12). Notably, as shown in Figure 1A, CD4+-naïve T lymphocytes after incubation with allogeneic MoDCs from colon carcinoma patients exhibited a significantly lower proliferative response (indicated by 3H-TdR uptake) (almost 10 times less) than did those stimulated with normal MoDCs (P ≤ .001). Colon carcinoma patients thus demonstrated a marked defect in DC functional activity.

Bottom Line: Because of their potent immunoregulatory capacities, DCs have been exploited in anticancer vaccination, with limited success thus far.No single agent enhanced the compromised allostimulatory activity of MoDCs from colon cancer patients, unlike healthy donors.However, MoDCs from nonmetastatic colon cancer patients, after sequential exposure to SKA-IL-4 (48 hours) and SKA-IL-12 (24 hours), displayed increased T-cell stimulatory capacity by MLR and acquired driving T-helper 1 polarization activity, although less markedly than the effects induced by recombinant human cytokines or found in normal subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Sciences, Corso Dogliotti 14, 10126 Turin, University of Turin, Italy. Electronic address: elisabetta.radice@unito.it.

No MeSH data available.


Related in: MedlinePlus