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Dasatinib Modulates Invasive and Migratory Properties of Canine Osteosarcoma and has Therapeutic Potential in Affected Dogs.

Marley K, Gullaba J, Seguin B, Gelberg HB, Helfand SC - Transl Oncol (2015)

Bottom Line: HGF induces secretion of different forms of MMP in different cell lines.The HGF-driven increase in viability and metastatic behaviors we observed are more uniformly inhibited by dasatinib.These observations suggest a potential clinical benefit of adjuvant dasatinib treatment for dogs with OS.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences Oregon State University, 105 Magruder Hall, Corvallis OR, 97331, USA. Electronic address: kevin.marley@oregonstate.edu.

No MeSH data available.


Related in: MedlinePlus

MET activation. (A) Western blots from serum-starved cells show that MET is constitutively activated in both cell lines. HGF incubation had little effect on MET phosphorylation at tyrosines 1230/1234/1235, and TKI incubation (20 nM) was not sufficient to reverse this. Dasatinib suppressed but did not eliminate the pY-MET signal in Clone-4 cells. (B) Histogram of densitometry of pY-MET Western blot shown in A.
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f0025: MET activation. (A) Western blots from serum-starved cells show that MET is constitutively activated in both cell lines. HGF incubation had little effect on MET phosphorylation at tyrosines 1230/1234/1235, and TKI incubation (20 nM) was not sufficient to reverse this. Dasatinib suppressed but did not eliminate the pY-MET signal in Clone-4 cells. (B) Histogram of densitometry of pY-MET Western blot shown in A.

Mentions: Tyrosine phosphorylation (pY) of MET in serum-starved cells was strongly positive and appeared unchanged by HGF (FigureĀ 5). TKI incubation similarly appeared to have little effect on pY-MET with the exception that dasatinib was able to reduce, but not eliminate, this activation signal in the Clone-4 cells.


Dasatinib Modulates Invasive and Migratory Properties of Canine Osteosarcoma and has Therapeutic Potential in Affected Dogs.

Marley K, Gullaba J, Seguin B, Gelberg HB, Helfand SC - Transl Oncol (2015)

MET activation. (A) Western blots from serum-starved cells show that MET is constitutively activated in both cell lines. HGF incubation had little effect on MET phosphorylation at tyrosines 1230/1234/1235, and TKI incubation (20 nM) was not sufficient to reverse this. Dasatinib suppressed but did not eliminate the pY-MET signal in Clone-4 cells. (B) Histogram of densitometry of pY-MET Western blot shown in A.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562978&req=5

f0025: MET activation. (A) Western blots from serum-starved cells show that MET is constitutively activated in both cell lines. HGF incubation had little effect on MET phosphorylation at tyrosines 1230/1234/1235, and TKI incubation (20 nM) was not sufficient to reverse this. Dasatinib suppressed but did not eliminate the pY-MET signal in Clone-4 cells. (B) Histogram of densitometry of pY-MET Western blot shown in A.
Mentions: Tyrosine phosphorylation (pY) of MET in serum-starved cells was strongly positive and appeared unchanged by HGF (FigureĀ 5). TKI incubation similarly appeared to have little effect on pY-MET with the exception that dasatinib was able to reduce, but not eliminate, this activation signal in the Clone-4 cells.

Bottom Line: HGF induces secretion of different forms of MMP in different cell lines.The HGF-driven increase in viability and metastatic behaviors we observed are more uniformly inhibited by dasatinib.These observations suggest a potential clinical benefit of adjuvant dasatinib treatment for dogs with OS.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences Oregon State University, 105 Magruder Hall, Corvallis OR, 97331, USA. Electronic address: kevin.marley@oregonstate.edu.

No MeSH data available.


Related in: MedlinePlus