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Hepatitis B Surface Antigen Quantification across Different Phases of Chronic Hepatitis B Virus Infection Using an Immunoradiometric Assay.

Chung KH, Kim W, Kim BG, Lee HY, Jin E, Cho Y, Seo JY, Kim HY, Jung YJ, Kim JW, Jeong JB, Lee KL - Gut Liver (2015)

Bottom Line: Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states.The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Quantification of hepatitis B surface antigen (HBsAg) is an emerging serologic test and may be useful for identifying treatment strategies for chronic hepatitis B (CHB). This study aimed to evaluate HBsAg titers during the natural course of CHB and identify correlations between HBsAg titers and hepatitis B virus (HBV) DNA concentrations across different CHB phases measured using an immunoradiometric assay (IRMA).

Methods: CHB phases were defined on the basis of HBV DNA concentrations, the presence of hepatitis B e antigen/antibody (HBeAg/Ab) and serum alanine aminotransferase levels. Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.

Results: Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states. The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

Conclusions: HBsAg quantification using IRMA might be useful for discriminating different CHB phases and different stages of chronic liver disease.

No MeSH data available.


Related in: MedlinePlus

Comparison of mean hepatitis B surface antigen (HBsAg) titers between patients with and without hepatocellular carcinoma.
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f5-gnl-09-657: Comparison of mean hepatitis B surface antigen (HBsAg) titers between patients with and without hepatocellular carcinoma.

Mentions: The mean serum HBsAg titers tended to be significantly higher in younger age groups than in older age groups. The mean serum HBsAg titers tended to gradually decrease as the 10-year age stratum increased (p for trend<0.001) (Fig. 3). The mean HBsAg titers significantly differed between the patients with and without cirrhosis. The mean HBsAg titer was nearly 5 times higher in the noncirrhosis group than in the cirrhosis group (21,517.6 IU/mL vs 4,532.8 IU/mL, p<0.001) (Fig. 4), and was significantly higher in the patients without HCC than in those with HCC (16,615.0 IU/mL vs 3,461.8 IU/mL, p<0.001) (Fig. 5). To control for the confounding effect of age on the HBsAg titers of those with liver cirrhosis and HCC, we performed subgroup analysis by stratifying patients’ age into <60 and ≥60. Of those who were younger than 60 year-old, the mean HBsAg titer was significantly higher in the noncirrhosis group than in the cirrhosis group (22,996.7 IU/mL vs 4,886.18 IU/mL, p<0.001) and was also significantly higher in the non-HCC group than in the HCC group (18,371.3 vs 3,443.7, p<0.001). Similarly, of those who were older than 60 year-old, the mean HBsAg titer was significantly higher in noncirrhotic patients than in cirrhotic ones (9,504.3 IU/mL vs 3,754.4 IU/mL, p=0.019) and was higher in the patients without HCC than in those with HCC, though it was not statistically significant (6,793.0 vs 3,645.6, p=0.12).


Hepatitis B Surface Antigen Quantification across Different Phases of Chronic Hepatitis B Virus Infection Using an Immunoradiometric Assay.

Chung KH, Kim W, Kim BG, Lee HY, Jin E, Cho Y, Seo JY, Kim HY, Jung YJ, Kim JW, Jeong JB, Lee KL - Gut Liver (2015)

Comparison of mean hepatitis B surface antigen (HBsAg) titers between patients with and without hepatocellular carcinoma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562784&req=5

f5-gnl-09-657: Comparison of mean hepatitis B surface antigen (HBsAg) titers between patients with and without hepatocellular carcinoma.
Mentions: The mean serum HBsAg titers tended to be significantly higher in younger age groups than in older age groups. The mean serum HBsAg titers tended to gradually decrease as the 10-year age stratum increased (p for trend<0.001) (Fig. 3). The mean HBsAg titers significantly differed between the patients with and without cirrhosis. The mean HBsAg titer was nearly 5 times higher in the noncirrhosis group than in the cirrhosis group (21,517.6 IU/mL vs 4,532.8 IU/mL, p<0.001) (Fig. 4), and was significantly higher in the patients without HCC than in those with HCC (16,615.0 IU/mL vs 3,461.8 IU/mL, p<0.001) (Fig. 5). To control for the confounding effect of age on the HBsAg titers of those with liver cirrhosis and HCC, we performed subgroup analysis by stratifying patients’ age into <60 and ≥60. Of those who were younger than 60 year-old, the mean HBsAg titer was significantly higher in the noncirrhosis group than in the cirrhosis group (22,996.7 IU/mL vs 4,886.18 IU/mL, p<0.001) and was also significantly higher in the non-HCC group than in the HCC group (18,371.3 vs 3,443.7, p<0.001). Similarly, of those who were older than 60 year-old, the mean HBsAg titer was significantly higher in noncirrhotic patients than in cirrhotic ones (9,504.3 IU/mL vs 3,754.4 IU/mL, p=0.019) and was higher in the patients without HCC than in those with HCC, though it was not statistically significant (6,793.0 vs 3,645.6, p=0.12).

Bottom Line: Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states.The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Quantification of hepatitis B surface antigen (HBsAg) is an emerging serologic test and may be useful for identifying treatment strategies for chronic hepatitis B (CHB). This study aimed to evaluate HBsAg titers during the natural course of CHB and identify correlations between HBsAg titers and hepatitis B virus (HBV) DNA concentrations across different CHB phases measured using an immunoradiometric assay (IRMA).

Methods: CHB phases were defined on the basis of HBV DNA concentrations, the presence of hepatitis B e antigen/antibody (HBeAg/Ab) and serum alanine aminotransferase levels. Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.

Results: Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states. The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

Conclusions: HBsAg quantification using IRMA might be useful for discriminating different CHB phases and different stages of chronic liver disease.

No MeSH data available.


Related in: MedlinePlus