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Hepatitis B Surface Antigen Quantification across Different Phases of Chronic Hepatitis B Virus Infection Using an Immunoradiometric Assay.

Chung KH, Kim W, Kim BG, Lee HY, Jin E, Cho Y, Seo JY, Kim HY, Jung YJ, Kim JW, Jeong JB, Lee KL - Gut Liver (2015)

Bottom Line: Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states.The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Quantification of hepatitis B surface antigen (HBsAg) is an emerging serologic test and may be useful for identifying treatment strategies for chronic hepatitis B (CHB). This study aimed to evaluate HBsAg titers during the natural course of CHB and identify correlations between HBsAg titers and hepatitis B virus (HBV) DNA concentrations across different CHB phases measured using an immunoradiometric assay (IRMA).

Methods: CHB phases were defined on the basis of HBV DNA concentrations, the presence of hepatitis B e antigen/antibody (HBeAg/Ab) and serum alanine aminotransferase levels. Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.

Results: Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states. The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

Conclusions: HBsAg quantification using IRMA might be useful for discriminating different CHB phases and different stages of chronic liver disease.

No MeSH data available.


Related in: MedlinePlus

Correlations between serum hepatitis B surface antigen (HBsAg) titers and hepatitis B virus (HBV) DNA concentrations in the different phases of chronic hepatitis B (CHB). (A) Total population; (B) immunotolerant phase (IT); (C) immunoreactive hepatitis B e antigen (HBeAg)-positive phase (IR); (D) low-replicative phase (LR); (E) HBeAg-negative CHB (ENH); (F) HBeAg-positive CHB with virologic response (E+VR); (G) HBeAg-positive CHB with partial virologic response (E+pVR); (H) HBeAg-negative CHB with virologic response (E-VR); (I) HBeAg-negative CHB with partial virologic response (E-pVR).
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f2-gnl-09-657: Correlations between serum hepatitis B surface antigen (HBsAg) titers and hepatitis B virus (HBV) DNA concentrations in the different phases of chronic hepatitis B (CHB). (A) Total population; (B) immunotolerant phase (IT); (C) immunoreactive hepatitis B e antigen (HBeAg)-positive phase (IR); (D) low-replicative phase (LR); (E) HBeAg-negative CHB (ENH); (F) HBeAg-positive CHB with virologic response (E+VR); (G) HBeAg-positive CHB with partial virologic response (E+pVR); (H) HBeAg-negative CHB with virologic response (E-VR); (I) HBeAg-negative CHB with partial virologic response (E-pVR).

Mentions: Correlations between HBsAg titers and HBV DNA concentrations are shown in Fig. 2. A modest correlation was observed in the IT and IR phases (r=0.804, p<0.001; and r=0.773, p<0.001). In addition, a poor correlation was observed in the LR phase (r=0.289, p=0.002). However, no significant correlation was observed in ENH (r=0.146, p=0.237), E+VR (r=0.082, p=0.588), E+pVR (r=0.194, p=0.083), E-VR (r=−0.009, p=0.940), or E-pVR (r=0.092, p=0.447).


Hepatitis B Surface Antigen Quantification across Different Phases of Chronic Hepatitis B Virus Infection Using an Immunoradiometric Assay.

Chung KH, Kim W, Kim BG, Lee HY, Jin E, Cho Y, Seo JY, Kim HY, Jung YJ, Kim JW, Jeong JB, Lee KL - Gut Liver (2015)

Correlations between serum hepatitis B surface antigen (HBsAg) titers and hepatitis B virus (HBV) DNA concentrations in the different phases of chronic hepatitis B (CHB). (A) Total population; (B) immunotolerant phase (IT); (C) immunoreactive hepatitis B e antigen (HBeAg)-positive phase (IR); (D) low-replicative phase (LR); (E) HBeAg-negative CHB (ENH); (F) HBeAg-positive CHB with virologic response (E+VR); (G) HBeAg-positive CHB with partial virologic response (E+pVR); (H) HBeAg-negative CHB with virologic response (E-VR); (I) HBeAg-negative CHB with partial virologic response (E-pVR).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562784&req=5

f2-gnl-09-657: Correlations between serum hepatitis B surface antigen (HBsAg) titers and hepatitis B virus (HBV) DNA concentrations in the different phases of chronic hepatitis B (CHB). (A) Total population; (B) immunotolerant phase (IT); (C) immunoreactive hepatitis B e antigen (HBeAg)-positive phase (IR); (D) low-replicative phase (LR); (E) HBeAg-negative CHB (ENH); (F) HBeAg-positive CHB with virologic response (E+VR); (G) HBeAg-positive CHB with partial virologic response (E+pVR); (H) HBeAg-negative CHB with virologic response (E-VR); (I) HBeAg-negative CHB with partial virologic response (E-pVR).
Mentions: Correlations between HBsAg titers and HBV DNA concentrations are shown in Fig. 2. A modest correlation was observed in the IT and IR phases (r=0.804, p<0.001; and r=0.773, p<0.001). In addition, a poor correlation was observed in the LR phase (r=0.289, p=0.002). However, no significant correlation was observed in ENH (r=0.146, p=0.237), E+VR (r=0.082, p=0.588), E+pVR (r=0.194, p=0.083), E-VR (r=−0.009, p=0.940), or E-pVR (r=0.092, p=0.447).

Bottom Line: Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states.The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Quantification of hepatitis B surface antigen (HBsAg) is an emerging serologic test and may be useful for identifying treatment strategies for chronic hepatitis B (CHB). This study aimed to evaluate HBsAg titers during the natural course of CHB and identify correlations between HBsAg titers and hepatitis B virus (HBV) DNA concentrations across different CHB phases measured using an immunoradiometric assay (IRMA).

Methods: CHB phases were defined on the basis of HBV DNA concentrations, the presence of hepatitis B e antigen/antibody (HBeAg/Ab) and serum alanine aminotransferase levels. Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.

Results: Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states. The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).

Conclusions: HBsAg quantification using IRMA might be useful for discriminating different CHB phases and different stages of chronic liver disease.

No MeSH data available.


Related in: MedlinePlus