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Biocompatible and biodegradable fibrinogen microspheres for tumor-targeted doxorubicin delivery.

Joo JY, Park GY, An SS - Int J Nanomedicine (2015)

Bottom Line: Doxorubicin (DOX), a chemotherapeutic agent, was covalently conjugated to Fbg, and the microspheres were prepared.In vitro cytotoxicity tests confirmed low cytotoxicity in normal cells and high antitumor effect toward cancer cells.Therefore, DOX-linker-Fbg microspheres could be a suitable drug carrier for safer and effective drug delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Seongnam-si, Republic of Korea.

ABSTRACT
In the development of effective drug delivery carriers, many researchers have focused on the usage of nontoxic and biocompatible materials and surface modification with targeting molecules for tumor-specific drug delivery. Fibrinogen (Fbg), an abundant glycoprotein in plasma, could be a potential candidate for developing drug carriers because of its biocompatibility and tumor-targeting property via arginine-glycine-aspartate (RGD) peptide sequences. Doxorubicin (DOX), a chemotherapeutic agent, was covalently conjugated to Fbg, and the microspheres were prepared. Acid-labile and non-cleavable linkers were used for the conjugation of DOX to Fbg, resulting in an acid-triggered drug release under a mild acidic condition and a slow-controlled drug release, respectively. In vitro cytotoxicity tests confirmed low cytotoxicity in normal cells and high antitumor effect toward cancer cells. In addition, it was discovered that a longer linker could make the binding of cells to Fbg drug carriers easier. Therefore, DOX-linker-Fbg microspheres could be a suitable drug carrier for safer and effective drug delivery.

No MeSH data available.


Related in: MedlinePlus

Confocal microscopic image of DOX–L1–Fbg microspheres.Abbreviations: DOX, doxorubicin; Fbg, fibrinogen; L1, linker 1.
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f2-ijn-10-101: Confocal microscopic image of DOX–L1–Fbg microspheres.Abbreviations: DOX, doxorubicin; Fbg, fibrinogen; L1, linker 1.

Mentions: Using a confocal microscope, it was revealed that DOX molecules were evenly distributed in the DOX–linker–Fbg microspheres. The bare-Fbg microspheres (without DOX loading) could not be observed by confocal microscope image, whereas the other three kinds of microspheres clearly showed DOX loading in the form of red-colored microspheres with similar shape as DOX–3-MAH–Fbg microspheres, as shown in Figure 2.


Biocompatible and biodegradable fibrinogen microspheres for tumor-targeted doxorubicin delivery.

Joo JY, Park GY, An SS - Int J Nanomedicine (2015)

Confocal microscopic image of DOX–L1–Fbg microspheres.Abbreviations: DOX, doxorubicin; Fbg, fibrinogen; L1, linker 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562758&req=5

f2-ijn-10-101: Confocal microscopic image of DOX–L1–Fbg microspheres.Abbreviations: DOX, doxorubicin; Fbg, fibrinogen; L1, linker 1.
Mentions: Using a confocal microscope, it was revealed that DOX molecules were evenly distributed in the DOX–linker–Fbg microspheres. The bare-Fbg microspheres (without DOX loading) could not be observed by confocal microscope image, whereas the other three kinds of microspheres clearly showed DOX loading in the form of red-colored microspheres with similar shape as DOX–3-MAH–Fbg microspheres, as shown in Figure 2.

Bottom Line: Doxorubicin (DOX), a chemotherapeutic agent, was covalently conjugated to Fbg, and the microspheres were prepared.In vitro cytotoxicity tests confirmed low cytotoxicity in normal cells and high antitumor effect toward cancer cells.Therefore, DOX-linker-Fbg microspheres could be a suitable drug carrier for safer and effective drug delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Seongnam-si, Republic of Korea.

ABSTRACT
In the development of effective drug delivery carriers, many researchers have focused on the usage of nontoxic and biocompatible materials and surface modification with targeting molecules for tumor-specific drug delivery. Fibrinogen (Fbg), an abundant glycoprotein in plasma, could be a potential candidate for developing drug carriers because of its biocompatibility and tumor-targeting property via arginine-glycine-aspartate (RGD) peptide sequences. Doxorubicin (DOX), a chemotherapeutic agent, was covalently conjugated to Fbg, and the microspheres were prepared. Acid-labile and non-cleavable linkers were used for the conjugation of DOX to Fbg, resulting in an acid-triggered drug release under a mild acidic condition and a slow-controlled drug release, respectively. In vitro cytotoxicity tests confirmed low cytotoxicity in normal cells and high antitumor effect toward cancer cells. In addition, it was discovered that a longer linker could make the binding of cells to Fbg drug carriers easier. Therefore, DOX-linker-Fbg microspheres could be a suitable drug carrier for safer and effective drug delivery.

No MeSH data available.


Related in: MedlinePlus