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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus

AFM topological images and cross-sectional surface profiles of titanium surfaces coated with (A) 0.25%, (B) 0.5%, and (C) 3% phosphonated gelatin. Each cross-sectional surface profile (bottom) was taken at the red line in the corresponding topological image.Abbreviation: AFM, atomic force microscope.
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f8-ijn-10-5597: AFM topological images and cross-sectional surface profiles of titanium surfaces coated with (A) 0.25%, (B) 0.5%, and (C) 3% phosphonated gelatin. Each cross-sectional surface profile (bottom) was taken at the red line in the corresponding topological image.Abbreviation: AFM, atomic force microscope.

Mentions: AFM observations indicated that a rough surface was formed by the bound gelatin (Figure 8). The roughness presented insignificant difference between different concentration (Ra =1.4, 1.1, and 1.4 nm for gelatin concentration of 0.25%, 0.5%, and 3%, respectively). The images show particle-like structures with a diameter of a few to several tens of nm. This observation suggests that, although the solution of phosphonated gelatin was transparent at room temperature, some nanoscale gel (nanogel) formed on the surface. However, the particles seem not fully covering the surface, which can also be seen from the cross-sectional profiles in Figure 8. In addition, surface roughness (1.1–1.4 nm) is slightly smaller than the thickness obtained by ellipsometry (~2.5 nm as shown in Figure 9). Although the thickness is not a precise film thickness but an averaged one, these results indicate that the gelatin forms fully covering layer on the substrate, on top of which some nanogel particles exists. The thickness of ~2.5 nm obtained by ellipsometry was on the same order of magnitude as the QCM results.


Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

AFM topological images and cross-sectional surface profiles of titanium surfaces coated with (A) 0.25%, (B) 0.5%, and (C) 3% phosphonated gelatin. Each cross-sectional surface profile (bottom) was taken at the red line in the corresponding topological image.Abbreviation: AFM, atomic force microscope.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562736&req=5

f8-ijn-10-5597: AFM topological images and cross-sectional surface profiles of titanium surfaces coated with (A) 0.25%, (B) 0.5%, and (C) 3% phosphonated gelatin. Each cross-sectional surface profile (bottom) was taken at the red line in the corresponding topological image.Abbreviation: AFM, atomic force microscope.
Mentions: AFM observations indicated that a rough surface was formed by the bound gelatin (Figure 8). The roughness presented insignificant difference between different concentration (Ra =1.4, 1.1, and 1.4 nm for gelatin concentration of 0.25%, 0.5%, and 3%, respectively). The images show particle-like structures with a diameter of a few to several tens of nm. This observation suggests that, although the solution of phosphonated gelatin was transparent at room temperature, some nanoscale gel (nanogel) formed on the surface. However, the particles seem not fully covering the surface, which can also be seen from the cross-sectional profiles in Figure 8. In addition, surface roughness (1.1–1.4 nm) is slightly smaller than the thickness obtained by ellipsometry (~2.5 nm as shown in Figure 9). Although the thickness is not a precise film thickness but an averaged one, these results indicate that the gelatin forms fully covering layer on the substrate, on top of which some nanogel particles exists. The thickness of ~2.5 nm obtained by ellipsometry was on the same order of magnitude as the QCM results.

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus