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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus

Negative ToF-SIMS results in the (A) 200.5–212.5 m/z, (B) 218.5–228.5 m/z, and (C) 233.5–243.5 m/z region of the phosphonated gelatin-coated titanium surface.Abbreviations: ToF-SIMS, time-of-flight secondary ion mass spectrometry; amu bin, binding energy per atom mass unit.
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f6-ijn-10-5597: Negative ToF-SIMS results in the (A) 200.5–212.5 m/z, (B) 218.5–228.5 m/z, and (C) 233.5–243.5 m/z region of the phosphonated gelatin-coated titanium surface.Abbreviations: ToF-SIMS, time-of-flight secondary ion mass spectrometry; amu bin, binding energy per atom mass unit.

Mentions: To investigate direct bonding between titanium and phosphonate groups in the modified gelatin, ToF-SIMS measurements were obtained as shown in Figure 6. Peaks ascribed to Ti-P2-O6, Ti-P2-O7H, and Ti-P3-O6H2 were detected. The phosphonated gelatin preferentially adsorbed to the titanium surface through direct bonding. The phosphonate in gelatin was considered to be concentrated at the interface with titanium. Similarly, Adden et al32 and Viornery et al33 also investigated the interactions of phosphonic acid linked to organic compounds with titanium by ToF-SIMS, and found strong indications of the formation of a chemical link, such as a Ti-O-P bond, between titanium and phosphonic acid molecules. According to Hotchkiss et al50 monolayers formed by phosphonic acids on metal oxide are more resistant to hydrolysis than those formed by silanes or carboxylic acids. The presence of three oxygen atoms is considered to allow covalent binding of phosphonic acid to an oxide surface in either monodentate, bidentate, or tridentate modes.50


Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Negative ToF-SIMS results in the (A) 200.5–212.5 m/z, (B) 218.5–228.5 m/z, and (C) 233.5–243.5 m/z region of the phosphonated gelatin-coated titanium surface.Abbreviations: ToF-SIMS, time-of-flight secondary ion mass spectrometry; amu bin, binding energy per atom mass unit.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562736&req=5

f6-ijn-10-5597: Negative ToF-SIMS results in the (A) 200.5–212.5 m/z, (B) 218.5–228.5 m/z, and (C) 233.5–243.5 m/z region of the phosphonated gelatin-coated titanium surface.Abbreviations: ToF-SIMS, time-of-flight secondary ion mass spectrometry; amu bin, binding energy per atom mass unit.
Mentions: To investigate direct bonding between titanium and phosphonate groups in the modified gelatin, ToF-SIMS measurements were obtained as shown in Figure 6. Peaks ascribed to Ti-P2-O6, Ti-P2-O7H, and Ti-P3-O6H2 were detected. The phosphonated gelatin preferentially adsorbed to the titanium surface through direct bonding. The phosphonate in gelatin was considered to be concentrated at the interface with titanium. Similarly, Adden et al32 and Viornery et al33 also investigated the interactions of phosphonic acid linked to organic compounds with titanium by ToF-SIMS, and found strong indications of the formation of a chemical link, such as a Ti-O-P bond, between titanium and phosphonic acid molecules. According to Hotchkiss et al50 monolayers formed by phosphonic acids on metal oxide are more resistant to hydrolysis than those formed by silanes or carboxylic acids. The presence of three oxygen atoms is considered to allow covalent binding of phosphonic acid to an oxide surface in either monodentate, bidentate, or tridentate modes.50

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus