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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus

Temperature-dependent turbidity of solutions of unmodified and phosphonated gelatin.Note: Wavelength: 600 nm, n=3.
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f5-ijn-10-5597: Temperature-dependent turbidity of solutions of unmodified and phosphonated gelatin.Note: Wavelength: 600 nm, n=3.

Mentions: Interestingly, the gelation behavior of phosphonated gelatin was very different from that of unmodified gelatin. As shown in Figure 4, when the temperature decreased from 37°C to 25°C, 3% unmodified gelatin solution formed gel, however, the 3% phosphonated gelatin remained the solution state. As shown in Figure 5, unmodified gelatin became turbid below 32.5°C, while the modified gelatin was transparent and did not form a gel even below 25°C. Incorporation of a small amount of phosphonic acid led to a drastic change in the gelatin temperature. It is known that the low gelation temperature of fish gelatin is due to the low content of proline and hydroxyproline.49 In this study, the incorporation of strong acidic groups (phosphonic acid) was considered to reduce the gelation temperature.


Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Temperature-dependent turbidity of solutions of unmodified and phosphonated gelatin.Note: Wavelength: 600 nm, n=3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562736&req=5

f5-ijn-10-5597: Temperature-dependent turbidity of solutions of unmodified and phosphonated gelatin.Note: Wavelength: 600 nm, n=3.
Mentions: Interestingly, the gelation behavior of phosphonated gelatin was very different from that of unmodified gelatin. As shown in Figure 4, when the temperature decreased from 37°C to 25°C, 3% unmodified gelatin solution formed gel, however, the 3% phosphonated gelatin remained the solution state. As shown in Figure 5, unmodified gelatin became turbid below 32.5°C, while the modified gelatin was transparent and did not form a gel even below 25°C. Incorporation of a small amount of phosphonic acid led to a drastic change in the gelatin temperature. It is known that the low gelation temperature of fish gelatin is due to the low content of proline and hydroxyproline.49 In this study, the incorporation of strong acidic groups (phosphonic acid) was considered to reduce the gelation temperature.

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus