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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus

31P NMR spectra of 3-aminopropylphosphonic acid and phosphonated gelatin in the presence of phosphoric acid as the standard (0 ppm).Abbreviation:31P NMR, 31P nuclear magnetic resonance.
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f2-ijn-10-5597: 31P NMR spectra of 3-aminopropylphosphonic acid and phosphonated gelatin in the presence of phosphoric acid as the standard (0 ppm).Abbreviation:31P NMR, 31P nuclear magnetic resonance.

Mentions: Figure 2 shows the 31P peaks of the uncoupled phosphonic acid and phosphonated gelatin. Both peaks were located between 15 and 30 ppm where phosphonate groups are ascribable.46 The peak of 3-aminopropylphosphonic acid observed at less than 25 ppm shifted to a higher peak by about 2 ppm after incorporation into gelatin.


Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

31P NMR spectra of 3-aminopropylphosphonic acid and phosphonated gelatin in the presence of phosphoric acid as the standard (0 ppm).Abbreviation:31P NMR, 31P nuclear magnetic resonance.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562736&req=5

f2-ijn-10-5597: 31P NMR spectra of 3-aminopropylphosphonic acid and phosphonated gelatin in the presence of phosphoric acid as the standard (0 ppm).Abbreviation:31P NMR, 31P nuclear magnetic resonance.
Mentions: Figure 2 shows the 31P peaks of the uncoupled phosphonic acid and phosphonated gelatin. Both peaks were located between 15 and 30 ppm where phosphonate groups are ascribable.46 The peak of 3-aminopropylphosphonic acid observed at less than 25 ppm shifted to a higher peak by about 2 ppm after incorporation into gelatin.

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus