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Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus

Micrographs of adhered cells on the phosphonated gelatin-coated titanium surface.
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f11-ijn-10-5597: Micrographs of adhered cells on the phosphonated gelatin-coated titanium surface.

Mentions: Figure 11 shows cells adhered on the uncoated and phosphonated gelatin-coated titanium surface. Using titanium-deposited glass discs, it was possible to observe the adhered cells by optical microscopy. The phosphonated gelatin-coated surface increased the spreading of cells over time. Figure 12 shows the number of adhered cells, as well as cell spreading and growth on the phosphonated gelatin-treated titanium surfaces. The spreading and growth on bare titanium discs was regarded 100%. The results indicated that phosphonation did not reduce the capacity of gelatin to support cell adhesion and spreading, and the enhancement of cell adhesion and spreading increased with the increase in the concentration of bound phosphonated gelatin on the surface. Lim et al38 also reported that conjugation of gelatin increases the adhesion and spreading of cells on hydrogel surfaces. Hou et al51 also showed a significant enhancement of endothelial cell adhesion and proliferation on gelatin-modified surfaces. It is known that the surface hydrophilicity does not directly relate with cell attachment. Ikada52 reported moderate hydrophilicity was the best for cell attachment. For example, very hydrophilic surface like hydrogel reduces cell attachment. The gelatin modification is considered to provide moderate hydrophilicity on the surface. Taken together, these results indicated that the phosphonation enhanced the binding of gelatin onto titanium without losing the original cell adhesion or growth enhancement properties.


Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement.

Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y - Int J Nanomedicine (2015)

Micrographs of adhered cells on the phosphonated gelatin-coated titanium surface.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562736&req=5

f11-ijn-10-5597: Micrographs of adhered cells on the phosphonated gelatin-coated titanium surface.
Mentions: Figure 11 shows cells adhered on the uncoated and phosphonated gelatin-coated titanium surface. Using titanium-deposited glass discs, it was possible to observe the adhered cells by optical microscopy. The phosphonated gelatin-coated surface increased the spreading of cells over time. Figure 12 shows the number of adhered cells, as well as cell spreading and growth on the phosphonated gelatin-treated titanium surfaces. The spreading and growth on bare titanium discs was regarded 100%. The results indicated that phosphonation did not reduce the capacity of gelatin to support cell adhesion and spreading, and the enhancement of cell adhesion and spreading increased with the increase in the concentration of bound phosphonated gelatin on the surface. Lim et al38 also reported that conjugation of gelatin increases the adhesion and spreading of cells on hydrogel surfaces. Hou et al51 also showed a significant enhancement of endothelial cell adhesion and proliferation on gelatin-modified surfaces. It is known that the surface hydrophilicity does not directly relate with cell attachment. Ikada52 reported moderate hydrophilicity was the best for cell attachment. For example, very hydrophilic surface like hydrogel reduces cell attachment. The gelatin modification is considered to provide moderate hydrophilicity on the surface. Taken together, these results indicated that the phosphonation enhanced the binding of gelatin onto titanium without losing the original cell adhesion or growth enhancement properties.

Bottom Line: Even a high concentration of modified gelatin did not form a gel at room temperature.Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium.Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

View Article: PubMed Central - PubMed

Affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People's Republic of China.

ABSTRACT
Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by (31)P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface.

No MeSH data available.


Related in: MedlinePlus