Active autophagy but not lipophagy in macrophages with defective lipolysis.
Bottom Line: We therefore generated mice lacking both ATGL and HSL (A0H0).Markedly decreased acid TG hydrolase activity and lipid flux independent of bafilomycin A1 treatment, however, argue against effective lysosomal degradation of LDs in A0H0 macrophages.We conclude that autophagy of proteins and cell organelles but not of LDs is active as a compensatory mechanism to circumvent and balance the reduced availability of energy substrates in A0H0 macrophages.
Affiliation: Institute of Molecular Biology & Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21, 8010 Graz, Austria.Show MeSH
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Mentions: To investigate the role of autophagy in LD turnover in macrophages, we loaded macrophages with [3H]OA-BSA and analyzed the flux in the absence and presence of bafilomycin A1. In A0 and A0H0 macrophages, there was a trend toward decreased FA release into the medium after 14 h of bafilomycin A1 treatment. However, we failed to observe any differences in untreated compared to bafilomycin A1-treated cells (Fig. 6A). Furthermore, we found increased FA incorporation into TGs in A0 and A0H0 macrophages, which was unaffected by bafilomycin A1 treatment (Fig. 6B). We observed no differences in incorporation of [3H]OA-BSA into other lipid classes between the different genotypes or after bafilomycin A1 incubation. Next, we treated Wt cells with inhibitors against ATGL (Ai) and HSL (Hi), resulting in significantly decreased FA release (Fig. 6C). This finding suggests that the inhibitors efficiently blocked lipolysis. Comparable to the results from A0 and A0H0 macrophages, we observed increased FA incorporation into TGs of inhibitor-treated Wt cells, which was unaffected by bafilomycin A1 treatment (Fig. 6D). In summary, results from these pulse-chase experiments revealed that incorporation of FAs into lipid classes was independent of bafilomycin A1 in macrophages from all genotypes.
Affiliation: Institute of Molecular Biology & Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21, 8010 Graz, Austria.