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Feasibility of up-regulating CD4(+)CD25(+) Tregs by IFN-γ in myasthenia gravis patients.

Huang S, Wang W, Chi L - BMC Neurol (2015)

Bottom Line: It shows the percentages of CD4(+)CD25(+) T cells among CD4(+) T cells have no significant difference in MG patients compared with those in HCs.This subject will further reveal the role of IFN-γ in the pathogenesis of MG from a new perspective.It will also provide the scientific basis for the clinical targeted therapy of MG.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150080, P.R. China. cecile_huang@163.com.

ABSTRACT

Background: In myasthenia gravis (MG) patients, the dysfunction of CD4(+)CD25(+) regulatory T cells (CD4(+)CD25(+) Tregs) may be one of the important pathogenesis of MG. Currently, the role of IFN-γ in autoimmune diseases is still controversial and needs further exploration. In this study, whether IFN-γ can induce CD4(+)CD25(-) T cells into CD4(+)CD25(+) Tregs in MG in vitro was investigated systematically.

Methods: Flow cytometry was used to analyze the number of CD4(+)CD25(+) Tregs in MG patients and healthy controls (HCs). CD4(+)CD25(-) T cells were separated from the peripheral blood mononuclear cells of MG patients and HCs, and the CD4(+)CD25(+) Tregs were separated from HCs by Magnetic cell sorting (MACS). IFN-γ with different concentrations was used to stimulate CD4(+)CD25(-) T cells. The percentages of the induced CD4(+)CD25(+) T cells were detected by flow cytometry. The FoxP3 expression of the induced CD4(+)CD25(+) T cells in MG patients was detected by real-time PCR at mRNA level. The induced CD4(+)CD25(+) T cells were co-cultured with autologous CD4(+)CD25(-) T cells to estimate the suppressive ability of the induced CD4(+)CD25(+) T cells to CD4(+)CD25(-) T cells.

Results: It shows the percentages of CD4(+)CD25(+) T cells among CD4(+) T cells have no significant difference in MG patients compared with those in HCs. There is also merely no difference in the percentages of CD4(+)CD25(+) T cells between thymectomized and non-thymectomized MG patients. CD4(+)CD25(-) T cells can be induced to CD4(+)CD25(+) T cells after applying IFN-γ in MG patients and HCs. The proportion and FoxP3 expression of the induced CD4(+)CD25(+) T cells are the highest at the level of 40 ng/ml IFN-γ, and the suppressive function of the CD4(+)CD25(+) T cells induced by 40 ng/ml IFN-γ is the strongest in MG patients.

Conclusions: This subject will further reveal the role of IFN-γ in the pathogenesis of MG from a new perspective. It will also provide the scientific basis for the clinical targeted therapy of MG.

No MeSH data available.


Related in: MedlinePlus

Comparation of the percentage of CD4+CD25+ Tregs between thymectomy and non- thymectomy MG patients
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Fig2: Comparation of the percentage of CD4+CD25+ Tregs between thymectomy and non- thymectomy MG patients

Mentions: Twenty three MG patients were divided into two groups, including thymectomized and non-thymectomized MG patients. The influence of thymectomized on the number of CD4+CD25+ Tregs was investigated by comparing the percentages of CD4+CD25+ Tregs in CD4+ T cells between two groups. The results exhibit that the percentage of CD4+CD25+ Tregs is 7.99 ± 1.92 % in thymectomized group, while it is 8.71 ± 2.11 % in non-thymectomized group. It can be found that there is no statistically significant difference between them (P > 0.05) (Fig. 2).Fig. 2


Feasibility of up-regulating CD4(+)CD25(+) Tregs by IFN-γ in myasthenia gravis patients.

Huang S, Wang W, Chi L - BMC Neurol (2015)

Comparation of the percentage of CD4+CD25+ Tregs between thymectomy and non- thymectomy MG patients
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562356&req=5

Fig2: Comparation of the percentage of CD4+CD25+ Tregs between thymectomy and non- thymectomy MG patients
Mentions: Twenty three MG patients were divided into two groups, including thymectomized and non-thymectomized MG patients. The influence of thymectomized on the number of CD4+CD25+ Tregs was investigated by comparing the percentages of CD4+CD25+ Tregs in CD4+ T cells between two groups. The results exhibit that the percentage of CD4+CD25+ Tregs is 7.99 ± 1.92 % in thymectomized group, while it is 8.71 ± 2.11 % in non-thymectomized group. It can be found that there is no statistically significant difference between them (P > 0.05) (Fig. 2).Fig. 2

Bottom Line: It shows the percentages of CD4(+)CD25(+) T cells among CD4(+) T cells have no significant difference in MG patients compared with those in HCs.This subject will further reveal the role of IFN-γ in the pathogenesis of MG from a new perspective.It will also provide the scientific basis for the clinical targeted therapy of MG.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150080, P.R. China. cecile_huang@163.com.

ABSTRACT

Background: In myasthenia gravis (MG) patients, the dysfunction of CD4(+)CD25(+) regulatory T cells (CD4(+)CD25(+) Tregs) may be one of the important pathogenesis of MG. Currently, the role of IFN-γ in autoimmune diseases is still controversial and needs further exploration. In this study, whether IFN-γ can induce CD4(+)CD25(-) T cells into CD4(+)CD25(+) Tregs in MG in vitro was investigated systematically.

Methods: Flow cytometry was used to analyze the number of CD4(+)CD25(+) Tregs in MG patients and healthy controls (HCs). CD4(+)CD25(-) T cells were separated from the peripheral blood mononuclear cells of MG patients and HCs, and the CD4(+)CD25(+) Tregs were separated from HCs by Magnetic cell sorting (MACS). IFN-γ with different concentrations was used to stimulate CD4(+)CD25(-) T cells. The percentages of the induced CD4(+)CD25(+) T cells were detected by flow cytometry. The FoxP3 expression of the induced CD4(+)CD25(+) T cells in MG patients was detected by real-time PCR at mRNA level. The induced CD4(+)CD25(+) T cells were co-cultured with autologous CD4(+)CD25(-) T cells to estimate the suppressive ability of the induced CD4(+)CD25(+) T cells to CD4(+)CD25(-) T cells.

Results: It shows the percentages of CD4(+)CD25(+) T cells among CD4(+) T cells have no significant difference in MG patients compared with those in HCs. There is also merely no difference in the percentages of CD4(+)CD25(+) T cells between thymectomized and non-thymectomized MG patients. CD4(+)CD25(-) T cells can be induced to CD4(+)CD25(+) T cells after applying IFN-γ in MG patients and HCs. The proportion and FoxP3 expression of the induced CD4(+)CD25(+) T cells are the highest at the level of 40 ng/ml IFN-γ, and the suppressive function of the CD4(+)CD25(+) T cells induced by 40 ng/ml IFN-γ is the strongest in MG patients.

Conclusions: This subject will further reveal the role of IFN-γ in the pathogenesis of MG from a new perspective. It will also provide the scientific basis for the clinical targeted therapy of MG.

No MeSH data available.


Related in: MedlinePlus