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Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells.

Jiang YH, Sun W, Li W, Hu HZ, Zhou L, Jiang HH, Xu JX - BMC Complement Altern Med (2015)

Bottom Line: The effects of calycosin on LPS-induced changes in cell viability, apoptosis rate, cell migration, nitric oxide synthase (NOS), generationof intracellular reactive oxygen species (ROS) and cytoskeleton organization were determined.Calycosin improved cell viability, suppressed apoptosis and protected the cells from LPS-induced reduction in cell migration and generation of ROS, protein level of NOS at a comparable magnitude to that of Y27632 and valsartan.Calycosin are considered to be able to activate MLCP through promoting the generation of NO, decreasing PMLC, suppressing the cytoskeleton remodeling caused by activation of Rho/ROCK pathway and inhibiting AKT pathway by decreasing VEGF, VEGF R2 and PI3K level.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of Shandong University of Traditional Chinese Medicine, West Wenhua road #42, Jinan, Shandong, 250011, PR China. jiang_yuehua@hotmail.com.

ABSTRACT

Background: Dysfunction of vascular endothelium is implicated in many pathological situations. Cytoskeleton plays an importance role in vascular endothelial permeability barrier and inflammatory response. Many Chinese herbs have the endothelial protective effect, of which, "Astragalus membranaceus" is a highly valued herb for treatment of cardiovascular and renal diseases in traditional Chinese medicine, In this study, we tested whether calycosin-7-O-β-D-glucoside (Calycosin), a main effective monomer component of "Astragalus membranaceus", could protect endothelial cells from bacterial endotoxin (LPS)-induced cell injury.

Methods: Endothelial cell injury was induced by exposing human umbilical vein endothelial cells (HUVECs) to LPS. The effects of calycosin on LPS-induced changes in cell viability, apoptosis rate, cell migration, nitric oxide synthase (NOS), generationof intracellular reactive oxygen species (ROS) and cytoskeleton organization were determined. Microarray assay was employed to screen the possible gene expression change. Based on the results of microarray assay, the expression profile of genes involved in Rho/ROCK pathway and AKT pathway were further evaluated with quantitative real-time RT-PCR or western blot methods.

Results: Calycosin improved cell viability, suppressed apoptosis and protected the cells from LPS-induced reduction in cell migration and generation of ROS, protein level of NOS at a comparable magnitude to that of Y27632 and valsartan. Similar to Y27632 and valsartan, Calycosin, also neutralized LPS-induced actomyosin contraction and vinculin protein aggregation. Microarray assay, real-time PCR and western blot results revealed that LPS induced expression of FN, ITG A5, RhoA, PI3K (or PIP2 in western blotting), FAK, VEGF and VEGF R2, and inhibited expression of MLCP. We believed multiple pathways involved in the regulation of calycosin on HUVECs. Calycosin are considered to be able to activate MLCP through promoting the generation of NO, decreasing PMLC, suppressing the cytoskeleton remodeling caused by activation of Rho/ROCK pathway and inhibiting AKT pathway by decreasing VEGF, VEGF R2 and PI3K level.

Conclusion: Calycosin protected HUVEC from LPS-induced endothelial injury, possibly through suppression of Rho/ROCK pathway and regulation of AKT pathway.

No MeSH data available.


Related in: MedlinePlus

Effect of Calycosin on LPS-induced disorganization of cytoskeleton and morphological change of vinculin. Immunofluorescence assay was performed with phalloidin and anti-vinculin antibody. Cells were fixed and stained for F-actin to observe cytoskeleton remodeling (red), vinculin (green) and merged images are shown in yellow which means that Rho/ROCK pathway was activated. Scale bar = 25 μm
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Fig5: Effect of Calycosin on LPS-induced disorganization of cytoskeleton and morphological change of vinculin. Immunofluorescence assay was performed with phalloidin and anti-vinculin antibody. Cells were fixed and stained for F-actin to observe cytoskeleton remodeling (red), vinculin (green) and merged images are shown in yellow which means that Rho/ROCK pathway was activated. Scale bar = 25 μm

Mentions: Normally, cytoskeletal protein fibrous-actin (F-actin) was arranged circularly around the subapical region of HUVECs; vinculin protein was evenly localized at cell-to-cell contact region. However LPS treatment led to cell shrinkage. F-actin and vinculin were found to be condensed aggregates. The results suggested that LPS activated the endothelial cells and induced actomyosin contraction. In the presence of calycosin or Y27632, these changes were largely prevented (Fig. 5). It was also prevented by valsartan.Fig. 5


Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells.

Jiang YH, Sun W, Li W, Hu HZ, Zhou L, Jiang HH, Xu JX - BMC Complement Altern Med (2015)

Effect of Calycosin on LPS-induced disorganization of cytoskeleton and morphological change of vinculin. Immunofluorescence assay was performed with phalloidin and anti-vinculin antibody. Cells were fixed and stained for F-actin to observe cytoskeleton remodeling (red), vinculin (green) and merged images are shown in yellow which means that Rho/ROCK pathway was activated. Scale bar = 25 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562353&req=5

Fig5: Effect of Calycosin on LPS-induced disorganization of cytoskeleton and morphological change of vinculin. Immunofluorescence assay was performed with phalloidin and anti-vinculin antibody. Cells were fixed and stained for F-actin to observe cytoskeleton remodeling (red), vinculin (green) and merged images are shown in yellow which means that Rho/ROCK pathway was activated. Scale bar = 25 μm
Mentions: Normally, cytoskeletal protein fibrous-actin (F-actin) was arranged circularly around the subapical region of HUVECs; vinculin protein was evenly localized at cell-to-cell contact region. However LPS treatment led to cell shrinkage. F-actin and vinculin were found to be condensed aggregates. The results suggested that LPS activated the endothelial cells and induced actomyosin contraction. In the presence of calycosin or Y27632, these changes were largely prevented (Fig. 5). It was also prevented by valsartan.Fig. 5

Bottom Line: The effects of calycosin on LPS-induced changes in cell viability, apoptosis rate, cell migration, nitric oxide synthase (NOS), generationof intracellular reactive oxygen species (ROS) and cytoskeleton organization were determined.Calycosin improved cell viability, suppressed apoptosis and protected the cells from LPS-induced reduction in cell migration and generation of ROS, protein level of NOS at a comparable magnitude to that of Y27632 and valsartan.Calycosin are considered to be able to activate MLCP through promoting the generation of NO, decreasing PMLC, suppressing the cytoskeleton remodeling caused by activation of Rho/ROCK pathway and inhibiting AKT pathway by decreasing VEGF, VEGF R2 and PI3K level.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of Shandong University of Traditional Chinese Medicine, West Wenhua road #42, Jinan, Shandong, 250011, PR China. jiang_yuehua@hotmail.com.

ABSTRACT

Background: Dysfunction of vascular endothelium is implicated in many pathological situations. Cytoskeleton plays an importance role in vascular endothelial permeability barrier and inflammatory response. Many Chinese herbs have the endothelial protective effect, of which, "Astragalus membranaceus" is a highly valued herb for treatment of cardiovascular and renal diseases in traditional Chinese medicine, In this study, we tested whether calycosin-7-O-β-D-glucoside (Calycosin), a main effective monomer component of "Astragalus membranaceus", could protect endothelial cells from bacterial endotoxin (LPS)-induced cell injury.

Methods: Endothelial cell injury was induced by exposing human umbilical vein endothelial cells (HUVECs) to LPS. The effects of calycosin on LPS-induced changes in cell viability, apoptosis rate, cell migration, nitric oxide synthase (NOS), generationof intracellular reactive oxygen species (ROS) and cytoskeleton organization were determined. Microarray assay was employed to screen the possible gene expression change. Based on the results of microarray assay, the expression profile of genes involved in Rho/ROCK pathway and AKT pathway were further evaluated with quantitative real-time RT-PCR or western blot methods.

Results: Calycosin improved cell viability, suppressed apoptosis and protected the cells from LPS-induced reduction in cell migration and generation of ROS, protein level of NOS at a comparable magnitude to that of Y27632 and valsartan. Similar to Y27632 and valsartan, Calycosin, also neutralized LPS-induced actomyosin contraction and vinculin protein aggregation. Microarray assay, real-time PCR and western blot results revealed that LPS induced expression of FN, ITG A5, RhoA, PI3K (or PIP2 in western blotting), FAK, VEGF and VEGF R2, and inhibited expression of MLCP. We believed multiple pathways involved in the regulation of calycosin on HUVECs. Calycosin are considered to be able to activate MLCP through promoting the generation of NO, decreasing PMLC, suppressing the cytoskeleton remodeling caused by activation of Rho/ROCK pathway and inhibiting AKT pathway by decreasing VEGF, VEGF R2 and PI3K level.

Conclusion: Calycosin protected HUVEC from LPS-induced endothelial injury, possibly through suppression of Rho/ROCK pathway and regulation of AKT pathway.

No MeSH data available.


Related in: MedlinePlus