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Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

Inhibition of Asparagus radix (ACE) on LPS-induced airway inflammation in mice. LPS was administered to mice intranasally. Total cell numbers in the BALF were counted using haemocytometer. The BALF was obtained at 16 h after LPS treatment. DEX: dexamethasone; +P < 0.1, ∗P < 0.05, and ∗∗P < 0.01, significantly different from the LPS-treated group (n = 5).
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fig5: Inhibition of Asparagus radix (ACE) on LPS-induced airway inflammation in mice. LPS was administered to mice intranasally. Total cell numbers in the BALF were counted using haemocytometer. The BALF was obtained at 16 h after LPS treatment. DEX: dexamethasone; +P < 0.1, ∗P < 0.05, and ∗∗P < 0.01, significantly different from the LPS-treated group (n = 5).

Mentions: A mouse model of airway inflammation induced by intranasal LPS treatment (acute lung injury) is used. In the present study, ACE showed inhibitory activity on this animal model. When administered orally at 100 and 400 mg/kg, ACE significantly reduced the number of total cells in the BALF (53.9% and 48.1% reduction, resp.), an important marker of lung inflammation (Figure 5).


Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Inhibition of Asparagus radix (ACE) on LPS-induced airway inflammation in mice. LPS was administered to mice intranasally. Total cell numbers in the BALF were counted using haemocytometer. The BALF was obtained at 16 h after LPS treatment. DEX: dexamethasone; +P < 0.1, ∗P < 0.05, and ∗∗P < 0.01, significantly different from the LPS-treated group (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562339&req=5

fig5: Inhibition of Asparagus radix (ACE) on LPS-induced airway inflammation in mice. LPS was administered to mice intranasally. Total cell numbers in the BALF were counted using haemocytometer. The BALF was obtained at 16 h after LPS treatment. DEX: dexamethasone; +P < 0.1, ∗P < 0.05, and ∗∗P < 0.01, significantly different from the LPS-treated group (n = 5).
Mentions: A mouse model of airway inflammation induced by intranasal LPS treatment (acute lung injury) is used. In the present study, ACE showed inhibitory activity on this animal model. When administered orally at 100 and 400 mg/kg, ACE significantly reduced the number of total cells in the BALF (53.9% and 48.1% reduction, resp.), an important marker of lung inflammation (Figure 5).

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus