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Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

Effects of Asparagus radix (ACE) and methyl protodioscin (MP) on iNOS-catalyzed NO production in MH-S cells. (a) Effects of ACE and MP against NO production. (b) Viability of MH-S cells measured by MTT assay. AMT: iNOS inhibitor and CT: cytotoxic; ∗P < 0.05, ∗∗P < 0.01, significantly different from the LPS-treated group (n = 3).
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fig4: Effects of Asparagus radix (ACE) and methyl protodioscin (MP) on iNOS-catalyzed NO production in MH-S cells. (a) Effects of ACE and MP against NO production. (b) Viability of MH-S cells measured by MTT assay. AMT: iNOS inhibitor and CT: cytotoxic; ∗P < 0.05, ∗∗P < 0.01, significantly different from the LPS-treated group (n = 3).

Mentions: Since NO is involved in lung inflammation, effects on NO production were examined. MH-S cells produce high amounts of NO by the stimulation of LPS via highly induced inducible NO synthase (iNOS) [19]. When MH-S cells were incubated with LPS for 24 h, NO concentration in the media increased to 44.9 ± 2.0 μM from the basal level of 1.1 ± 0.1 μM (n = 3) (Figure 4(a)). Under this condition, ACE did not inhibit NO production. On the other hand, MP showed a slight inhibitory effect on NO production (14.2%) only at 100 μM, but it showed some cytotoxic effect (6.6% cytotoxicity) on MH-S cells at this concentration (Figure 4(b)). NO-inhibitory action of MP might be related to its cytotoxicity on MH-S cells. Thus, it is thought that ACE and MP do not possess meaningful NO inhibitory activity.


Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Effects of Asparagus radix (ACE) and methyl protodioscin (MP) on iNOS-catalyzed NO production in MH-S cells. (a) Effects of ACE and MP against NO production. (b) Viability of MH-S cells measured by MTT assay. AMT: iNOS inhibitor and CT: cytotoxic; ∗P < 0.05, ∗∗P < 0.01, significantly different from the LPS-treated group (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562339&req=5

fig4: Effects of Asparagus radix (ACE) and methyl protodioscin (MP) on iNOS-catalyzed NO production in MH-S cells. (a) Effects of ACE and MP against NO production. (b) Viability of MH-S cells measured by MTT assay. AMT: iNOS inhibitor and CT: cytotoxic; ∗P < 0.05, ∗∗P < 0.01, significantly different from the LPS-treated group (n = 3).
Mentions: Since NO is involved in lung inflammation, effects on NO production were examined. MH-S cells produce high amounts of NO by the stimulation of LPS via highly induced inducible NO synthase (iNOS) [19]. When MH-S cells were incubated with LPS for 24 h, NO concentration in the media increased to 44.9 ± 2.0 μM from the basal level of 1.1 ± 0.1 μM (n = 3) (Figure 4(a)). Under this condition, ACE did not inhibit NO production. On the other hand, MP showed a slight inhibitory effect on NO production (14.2%) only at 100 μM, but it showed some cytotoxic effect (6.6% cytotoxicity) on MH-S cells at this concentration (Figure 4(b)). NO-inhibitory action of MP might be related to its cytotoxicity on MH-S cells. Thus, it is thought that ACE and MP do not possess meaningful NO inhibitory activity.

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus