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Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

Effects of the 70% ethanol extract of Asparagus radix (ACE) and methyl protodioscin (MP) on inflammatory cytokines/chemokine production in A549 cells. (a) Inhibition of ACE on IL-6 production. (b) Inhibition of MP on IL-6 production. (c) Inhibition of MP on TNF-α and IL-8 production. DEX: dexamethasone; ∗P < 0.05, ∗∗P < 0.01, significantly different from the IL-1β-treated group (n = 3).
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fig2: Effects of the 70% ethanol extract of Asparagus radix (ACE) and methyl protodioscin (MP) on inflammatory cytokines/chemokine production in A549 cells. (a) Inhibition of ACE on IL-6 production. (b) Inhibition of MP on IL-6 production. (c) Inhibition of MP on TNF-α and IL-8 production. DEX: dexamethasone; ∗P < 0.05, ∗∗P < 0.01, significantly different from the IL-1β-treated group (n = 3).

Mentions: For elucidating an inhibitory potential against lung inflammation, the effects on proinflammatory cytokine/chemokine generation in lung epithelial cells (A549) were examined. The cells were cultured and stimulated with IL-1β for inducing inflammatory responses. IL-1β treatment produced high amounts of IL-6 (0.42 ± 0.03 ng/mL) from A549 cells for 4 h in the media (n = 3) (Figure 2(a)). In this condition, the 70% ethanol extract of A. cochinchinensis (ACE) significantly inhibited IL-6 production at 100–500 μg/mL (41.0–63.8% inhibition, IC50 = 223.7 μg/mL). Dexamethasone (10 μM), which was used as a reference drug, showed potent inhibition (70.4%). According to this result, the major constituent was isolated from A. cochinchinensis. As shown in Figure 2(b), the isolated compound, methyl protodioscin (MP), strongly inhibited IL-6 production at 10–100 μM (IC50 = 59.1 μM). In addition, the concentrations of the other important proinflammatory cytokine (TNF-α) and chemokine (IL-8) were examined with the CBA method. MP also strongly inhibited TNF-α and IL-8 production (Figure 2(c)).


Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Effects of the 70% ethanol extract of Asparagus radix (ACE) and methyl protodioscin (MP) on inflammatory cytokines/chemokine production in A549 cells. (a) Inhibition of ACE on IL-6 production. (b) Inhibition of MP on IL-6 production. (c) Inhibition of MP on TNF-α and IL-8 production. DEX: dexamethasone; ∗P < 0.05, ∗∗P < 0.01, significantly different from the IL-1β-treated group (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562339&req=5

fig2: Effects of the 70% ethanol extract of Asparagus radix (ACE) and methyl protodioscin (MP) on inflammatory cytokines/chemokine production in A549 cells. (a) Inhibition of ACE on IL-6 production. (b) Inhibition of MP on IL-6 production. (c) Inhibition of MP on TNF-α and IL-8 production. DEX: dexamethasone; ∗P < 0.05, ∗∗P < 0.01, significantly different from the IL-1β-treated group (n = 3).
Mentions: For elucidating an inhibitory potential against lung inflammation, the effects on proinflammatory cytokine/chemokine generation in lung epithelial cells (A549) were examined. The cells were cultured and stimulated with IL-1β for inducing inflammatory responses. IL-1β treatment produced high amounts of IL-6 (0.42 ± 0.03 ng/mL) from A549 cells for 4 h in the media (n = 3) (Figure 2(a)). In this condition, the 70% ethanol extract of A. cochinchinensis (ACE) significantly inhibited IL-6 production at 100–500 μg/mL (41.0–63.8% inhibition, IC50 = 223.7 μg/mL). Dexamethasone (10 μM), which was used as a reference drug, showed potent inhibition (70.4%). According to this result, the major constituent was isolated from A. cochinchinensis. As shown in Figure 2(b), the isolated compound, methyl protodioscin (MP), strongly inhibited IL-6 production at 10–100 μM (IC50 = 59.1 μM). In addition, the concentrations of the other important proinflammatory cytokine (TNF-α) and chemokine (IL-8) were examined with the CBA method. MP also strongly inhibited TNF-α and IL-8 production (Figure 2(c)).

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus