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Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus

The chemical structure of methyl protodioscin.
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Related In: Results  -  Collection


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fig1: The chemical structure of methyl protodioscin.

Mentions: The dried roots of A. cochinchinensis (1 kg) were extracted with 70% ethanol under reflux. Evaporation under reduced pressure gave the dried extract (ACE, 55.18 g), which was used throughout the pharmacological study. For the isolation of the major compound, the dried roots of A. cochinchinensis (54.7 kg) were extracted with methanol at 60°C three times and the extract was evaporated under reduced pressure, yielding 1.41 kg of the methanol extract. The extract was successfully fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, and the fractions were evaporated to give 12.1 g, 67.0 g, 14.0 g, and 604.9 g, respectively. The n-butanol fraction (150.2 g) was loaded on a silica gel column and eluted with 100% hexane, methylene chloride : methanol, and methylene chloride : methanol : water, successfully, yielding 1–20 subfractions. Subfraction 19 was loaded on an RP-18 column and eluted with acetonitrile : water (2 : 8–5 : 5) to yield methyl protodioscin (MP, 1.64 g) (Figure 1). The physicochemical data including the 1H NMR and 13C NMR spectra of methyl protodioscin below were identical with those reported in the literature [17]. The content of MP in the ethanol extract and n-butanol fraction was found to be 0.2–0.5% and 12.5–30.0% (w/w), respectively, by HPLC analysis.


Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

Lee JH, Lim HJ, Lee CW, Son KH, Son JK, Lee SK, Kim HP - Evid Based Complement Alternat Med (2015)

The chemical structure of methyl protodioscin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562339&req=5

fig1: The chemical structure of methyl protodioscin.
Mentions: The dried roots of A. cochinchinensis (1 kg) were extracted with 70% ethanol under reflux. Evaporation under reduced pressure gave the dried extract (ACE, 55.18 g), which was used throughout the pharmacological study. For the isolation of the major compound, the dried roots of A. cochinchinensis (54.7 kg) were extracted with methanol at 60°C three times and the extract was evaporated under reduced pressure, yielding 1.41 kg of the methanol extract. The extract was successfully fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, and the fractions were evaporated to give 12.1 g, 67.0 g, 14.0 g, and 604.9 g, respectively. The n-butanol fraction (150.2 g) was loaded on a silica gel column and eluted with 100% hexane, methylene chloride : methanol, and methylene chloride : methanol : water, successfully, yielding 1–20 subfractions. Subfraction 19 was loaded on an RP-18 column and eluted with acetonitrile : water (2 : 8–5 : 5) to yield methyl protodioscin (MP, 1.64 g) (Figure 1). The physicochemical data including the 1H NMR and 13C NMR spectra of methyl protodioscin below were identical with those reported in the literature [17]. The content of MP in the ethanol extract and n-butanol fraction was found to be 0.2–0.5% and 12.5–30.0% (w/w), respectively, by HPLC analysis.

Bottom Line: This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway.When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively.All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.

ABSTRACT
The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

No MeSH data available.


Related in: MedlinePlus