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Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Wilson KS, Baily J, Tucker CS, Matrone G, Vass S, Moran C, Chapman KE, Mullins JJ, Kenyon C, Hadoke PW, Denvir MA - Mol. Cell. Endocrinol. (2015)

Bottom Line: GR Mo embryos (120 hpf) had smaller hearts with fewer cardiomyocytes, less mature striation pattern, reduced cardiac function and reduced levels of vmhc and igf mRNA compared with controls.GR Mo adult hearts were smaller with diminished trabecular network pattern, reduced expression of vmhc and altered echocardiographic Doppler flow compared to controls.Perturbations in GR activity during embryonic development results in short and long-term alterations in the heart.

View Article: PubMed Central - PubMed

Affiliation: The British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh EH16 4TJ, UK.

No MeSH data available.


Related in: MedlinePlus

Effects of embryonic glucocorticoid receptor manipulation on adult cardiac function Echocardiography was used to assess function of the adult ventricle using standard B-mode techniques and inflow and outflow Doppler patterns of the ventricle. A) Show typical examples of B-mode images used for calculation of ejection fraction using the epicardial outline in systole and diastole. B) Doppler signals obtained in the transverse plain were used to capture inflow and outflow velocity envelopes. Analysis of all images was undertaken by an independent operator blinded to treatment of the zebrafish. From echocardiography a number of cardiac functional parameters were determined following manipulation of glucocorticoid receptor (GR) such as C) heart rate, D) ejection fraction, E) end diastolic area, F) inflow mean velocity, G) inflow deceleration and H) inflow velocity time integral (vti). Data presented are for adults treated with 100 μM dexamethasone (Dex) as embryos n = 10, those treated with morpholino targeted towards Gr (GR Mo) n = 10, and Controls n = 17 (consisting of Dex controls n = 9 and GR Mo controls n = 8) and displayed as mean ± SEM. Data were analysed by one-way ANOVA and Dunnett's post hoc test, (*p < 0.05 and **p < 0.01).
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fig6: Effects of embryonic glucocorticoid receptor manipulation on adult cardiac function Echocardiography was used to assess function of the adult ventricle using standard B-mode techniques and inflow and outflow Doppler patterns of the ventricle. A) Show typical examples of B-mode images used for calculation of ejection fraction using the epicardial outline in systole and diastole. B) Doppler signals obtained in the transverse plain were used to capture inflow and outflow velocity envelopes. Analysis of all images was undertaken by an independent operator blinded to treatment of the zebrafish. From echocardiography a number of cardiac functional parameters were determined following manipulation of glucocorticoid receptor (GR) such as C) heart rate, D) ejection fraction, E) end diastolic area, F) inflow mean velocity, G) inflow deceleration and H) inflow velocity time integral (vti). Data presented are for adults treated with 100 μM dexamethasone (Dex) as embryos n = 10, those treated with morpholino targeted towards Gr (GR Mo) n = 10, and Controls n = 17 (consisting of Dex controls n = 9 and GR Mo controls n = 8) and displayed as mean ± SEM. Data were analysed by one-way ANOVA and Dunnett's post hoc test, (*p < 0.05 and **p < 0.01).

Mentions: Echocardiography (Fig. 6) indicated that adult hearts derived from GR Mo embryos had similar heart rate (Fig. 6C), ejection fraction (Fig. 6D) and end diastolic area (Fig. 6E) however had reduced ventricular inflow velocity (Fig. 6F), inflow deceleration time (Fig. 6G) and velocity time integral (Fig. 6H) compared to controls. Dex-treated adult hearts had similar ejection fraction and Doppler signals compared to controls (Fig. 6B–G).


Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Wilson KS, Baily J, Tucker CS, Matrone G, Vass S, Moran C, Chapman KE, Mullins JJ, Kenyon C, Hadoke PW, Denvir MA - Mol. Cell. Endocrinol. (2015)

Effects of embryonic glucocorticoid receptor manipulation on adult cardiac function Echocardiography was used to assess function of the adult ventricle using standard B-mode techniques and inflow and outflow Doppler patterns of the ventricle. A) Show typical examples of B-mode images used for calculation of ejection fraction using the epicardial outline in systole and diastole. B) Doppler signals obtained in the transverse plain were used to capture inflow and outflow velocity envelopes. Analysis of all images was undertaken by an independent operator blinded to treatment of the zebrafish. From echocardiography a number of cardiac functional parameters were determined following manipulation of glucocorticoid receptor (GR) such as C) heart rate, D) ejection fraction, E) end diastolic area, F) inflow mean velocity, G) inflow deceleration and H) inflow velocity time integral (vti). Data presented are for adults treated with 100 μM dexamethasone (Dex) as embryos n = 10, those treated with morpholino targeted towards Gr (GR Mo) n = 10, and Controls n = 17 (consisting of Dex controls n = 9 and GR Mo controls n = 8) and displayed as mean ± SEM. Data were analysed by one-way ANOVA and Dunnett's post hoc test, (*p < 0.05 and **p < 0.01).
© Copyright Policy - CC BY
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4562295&req=5

fig6: Effects of embryonic glucocorticoid receptor manipulation on adult cardiac function Echocardiography was used to assess function of the adult ventricle using standard B-mode techniques and inflow and outflow Doppler patterns of the ventricle. A) Show typical examples of B-mode images used for calculation of ejection fraction using the epicardial outline in systole and diastole. B) Doppler signals obtained in the transverse plain were used to capture inflow and outflow velocity envelopes. Analysis of all images was undertaken by an independent operator blinded to treatment of the zebrafish. From echocardiography a number of cardiac functional parameters were determined following manipulation of glucocorticoid receptor (GR) such as C) heart rate, D) ejection fraction, E) end diastolic area, F) inflow mean velocity, G) inflow deceleration and H) inflow velocity time integral (vti). Data presented are for adults treated with 100 μM dexamethasone (Dex) as embryos n = 10, those treated with morpholino targeted towards Gr (GR Mo) n = 10, and Controls n = 17 (consisting of Dex controls n = 9 and GR Mo controls n = 8) and displayed as mean ± SEM. Data were analysed by one-way ANOVA and Dunnett's post hoc test, (*p < 0.05 and **p < 0.01).
Mentions: Echocardiography (Fig. 6) indicated that adult hearts derived from GR Mo embryos had similar heart rate (Fig. 6C), ejection fraction (Fig. 6D) and end diastolic area (Fig. 6E) however had reduced ventricular inflow velocity (Fig. 6F), inflow deceleration time (Fig. 6G) and velocity time integral (Fig. 6H) compared to controls. Dex-treated adult hearts had similar ejection fraction and Doppler signals compared to controls (Fig. 6B–G).

Bottom Line: GR Mo embryos (120 hpf) had smaller hearts with fewer cardiomyocytes, less mature striation pattern, reduced cardiac function and reduced levels of vmhc and igf mRNA compared with controls.GR Mo adult hearts were smaller with diminished trabecular network pattern, reduced expression of vmhc and altered echocardiographic Doppler flow compared to controls.Perturbations in GR activity during embryonic development results in short and long-term alterations in the heart.

View Article: PubMed Central - PubMed

Affiliation: The British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh EH16 4TJ, UK.

No MeSH data available.


Related in: MedlinePlus