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Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Wilson KS, Baily J, Tucker CS, Matrone G, Vass S, Moran C, Chapman KE, Mullins JJ, Kenyon C, Hadoke PW, Denvir MA - Mol. Cell. Endocrinol. (2015)

Bottom Line: GR Mo embryos (120 hpf) had smaller hearts with fewer cardiomyocytes, less mature striation pattern, reduced cardiac function and reduced levels of vmhc and igf mRNA compared with controls.GR Mo adult hearts were smaller with diminished trabecular network pattern, reduced expression of vmhc and altered echocardiographic Doppler flow compared to controls.Perturbations in GR activity during embryonic development results in short and long-term alterations in the heart.

View Article: PubMed Central - PubMed

Affiliation: The British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh EH16 4TJ, UK.

No MeSH data available.


Related in: MedlinePlus

Manipulation of glucocorticoid activity induces changes in cardiac structure Confocal images of embryonic (120 h post fertilization (hpf)) of Tg (CMLC2: GFP) zebrafish hearts after 120 h glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone, (Dex) [100 μM] or targeted GR knockdown using morpholino (GR Mo). Whole mounted GFP embryonic hearts (green) were stained with DAPI (blue) and imaged at high power with a confocal microscope. Gross cardiac morphology can be observed at ×20 magnification, higher power images (×40 magnification) demonstrate advanced maturity of the Dex treated hearts in contrast to the GR Mo hearts which show considerable immaturity of myofibrillar and trabecular patterning. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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fig1: Manipulation of glucocorticoid activity induces changes in cardiac structure Confocal images of embryonic (120 h post fertilization (hpf)) of Tg (CMLC2: GFP) zebrafish hearts after 120 h glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone, (Dex) [100 μM] or targeted GR knockdown using morpholino (GR Mo). Whole mounted GFP embryonic hearts (green) were stained with DAPI (blue) and imaged at high power with a confocal microscope. Gross cardiac morphology can be observed at ×20 magnification, higher power images (×40 magnification) demonstrate advanced maturity of the Dex treated hearts in contrast to the GR Mo hearts which show considerable immaturity of myofibrillar and trabecular patterning. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Mentions: In contrast to control (age-matched and untreated) hearts at 120 hpf, which displayed a clear striation pattern with a mature trabecular network (Fig. 1), hearts from GR Mo embryos were smaller with a less well developed trabecular network and absent striations. Dex treated hearts, in contrast to the GR Mo hearts, showed a mature trabecular network with well-developed striation patterns.


Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Wilson KS, Baily J, Tucker CS, Matrone G, Vass S, Moran C, Chapman KE, Mullins JJ, Kenyon C, Hadoke PW, Denvir MA - Mol. Cell. Endocrinol. (2015)

Manipulation of glucocorticoid activity induces changes in cardiac structure Confocal images of embryonic (120 h post fertilization (hpf)) of Tg (CMLC2: GFP) zebrafish hearts after 120 h glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone, (Dex) [100 μM] or targeted GR knockdown using morpholino (GR Mo). Whole mounted GFP embryonic hearts (green) were stained with DAPI (blue) and imaged at high power with a confocal microscope. Gross cardiac morphology can be observed at ×20 magnification, higher power images (×40 magnification) demonstrate advanced maturity of the Dex treated hearts in contrast to the GR Mo hearts which show considerable immaturity of myofibrillar and trabecular patterning. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562295&req=5

fig1: Manipulation of glucocorticoid activity induces changes in cardiac structure Confocal images of embryonic (120 h post fertilization (hpf)) of Tg (CMLC2: GFP) zebrafish hearts after 120 h glucocorticoid receptor (GR) manipulation with either GR agonist dexamethasone, (Dex) [100 μM] or targeted GR knockdown using morpholino (GR Mo). Whole mounted GFP embryonic hearts (green) were stained with DAPI (blue) and imaged at high power with a confocal microscope. Gross cardiac morphology can be observed at ×20 magnification, higher power images (×40 magnification) demonstrate advanced maturity of the Dex treated hearts in contrast to the GR Mo hearts which show considerable immaturity of myofibrillar and trabecular patterning. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Mentions: In contrast to control (age-matched and untreated) hearts at 120 hpf, which displayed a clear striation pattern with a mature trabecular network (Fig. 1), hearts from GR Mo embryos were smaller with a less well developed trabecular network and absent striations. Dex treated hearts, in contrast to the GR Mo hearts, showed a mature trabecular network with well-developed striation patterns.

Bottom Line: GR Mo embryos (120 hpf) had smaller hearts with fewer cardiomyocytes, less mature striation pattern, reduced cardiac function and reduced levels of vmhc and igf mRNA compared with controls.GR Mo adult hearts were smaller with diminished trabecular network pattern, reduced expression of vmhc and altered echocardiographic Doppler flow compared to controls.Perturbations in GR activity during embryonic development results in short and long-term alterations in the heart.

View Article: PubMed Central - PubMed

Affiliation: The British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh EH16 4TJ, UK.

No MeSH data available.


Related in: MedlinePlus