Limits...
Species delimitation, genetic diversity and population historical dynamics of Cycas diannanensis (Cycadaceae) occurring sympatrically in the Red River region of China.

Liu J, Zhou W, Gong X - Front Plant Sci (2015)

Bottom Line: Wang.For operational guidelines, the downstream populations which occupy high and peculiar haplotypes should be given prior in-situ conservation.In addition, ex-situ conservation and reintroduction measures for decades of generations are supplemented for improving the population size and genetic diversity of the endemic and endangered species.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, China ; Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, China ; University of Chinese Academy of Sciences Beijing, China.

ABSTRACT
Delimitating species boundaries could be of critical importance when evaluating the species' evolving process and providing guidelines for conservation genetics. Here, species delimitation was carried out on three endemic and endangered Cycas species with resembling morphology and overlapped distribution range along the Red River (Yuanjiang) in China: Cycas diananensis Z. T. Guan et G. D. Tao, Cycas parvula S. L. Yang and Cycas multiovula D. Y. Wang. A total of 137 individuals from 15 populations were genotyped by using three chloroplastic (psbA-trnH, atpI-atpH, and trnL-rps4) and two single copy nuclear (RPB1 and SmHP) DNA sequences. Basing on the carefully morphological comparison and cladistic haplotype aggregation (CHA) analysis, we propose all the populations as one species, with the rest two incorporated into C. diannanensis. Genetic diversity and structure analysis of the conflated C. diannanensis revealed this species possessed a relative lower genetic diversity than estimates of other Cycas species. The higher genetic diversity among populations and relative lower genetic diversity within populations, as well as obvious genetic differentiation among populations inferred from chloroplastic DNA (cpDNA) suggested a recent genetic loss within this protected species. Additionally, a clear genetic structure of C. diannanensis corresponding with geography was detected based on cpDNA, dividing its population ranges into "Yuanjiang-Nanhun" basin and "Ejia-Jiepai" basin groups. Demographical history analyses based on combined cpDNA and one nuclear DNA (nDNA) SmHP both showed the population size of C. diannanensis began to decrease in Quaternary glaciation with no subsequent expansion, while another nDNA RPB1 revealed a more recent sudden expansion after long-term population size contraction, suggesting its probable bottleneck events in history. Our findings offer grounded views for clarifying species boundaries of C. diannanensis when determining the conservation objectives. For operational guidelines, the downstream populations which occupy high and peculiar haplotypes should be given prior in-situ conservation. In addition, ex-situ conservation and reintroduction measures for decades of generations are supplemented for improving the population size and genetic diversity of the endemic and endangered species.

No MeSH data available.


Related in: MedlinePlus

Geographical distributions of cpDNA haplotypes (A), nDNA SmHP haplotypes (B), nDNA RPB1 haplotypes (C) in C. diannanensis. Frequencies of haplotypes in each population are indicated by the proportions of pie diagrams. The colored dash areas present the sampling and possible distributions of the Cycas species in this study: Red, C. diannanensis; Green, C. parvula; Blue, C. multiovula.
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Figure 1: Geographical distributions of cpDNA haplotypes (A), nDNA SmHP haplotypes (B), nDNA RPB1 haplotypes (C) in C. diannanensis. Frequencies of haplotypes in each population are indicated by the proportions of pie diagrams. The colored dash areas present the sampling and possible distributions of the Cycas species in this study: Red, C. diannanensis; Green, C. parvula; Blue, C. multiovula.

Mentions: The combined chloroplastic sequence data of atpI-atpH, psbA-trnH, and trnL-rps4 was aligned as a consensus length of 1992 bp, containing 61 polymorphic sites among which 14 were substitutions and others were indels. A total of 13 haplotypes were detected in the 15 populations. The haplotype distributing patterns were listed in the Table S1 and showed in the Figure 1A.


Species delimitation, genetic diversity and population historical dynamics of Cycas diannanensis (Cycadaceae) occurring sympatrically in the Red River region of China.

Liu J, Zhou W, Gong X - Front Plant Sci (2015)

Geographical distributions of cpDNA haplotypes (A), nDNA SmHP haplotypes (B), nDNA RPB1 haplotypes (C) in C. diannanensis. Frequencies of haplotypes in each population are indicated by the proportions of pie diagrams. The colored dash areas present the sampling and possible distributions of the Cycas species in this study: Red, C. diannanensis; Green, C. parvula; Blue, C. multiovula.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562272&req=5

Figure 1: Geographical distributions of cpDNA haplotypes (A), nDNA SmHP haplotypes (B), nDNA RPB1 haplotypes (C) in C. diannanensis. Frequencies of haplotypes in each population are indicated by the proportions of pie diagrams. The colored dash areas present the sampling and possible distributions of the Cycas species in this study: Red, C. diannanensis; Green, C. parvula; Blue, C. multiovula.
Mentions: The combined chloroplastic sequence data of atpI-atpH, psbA-trnH, and trnL-rps4 was aligned as a consensus length of 1992 bp, containing 61 polymorphic sites among which 14 were substitutions and others were indels. A total of 13 haplotypes were detected in the 15 populations. The haplotype distributing patterns were listed in the Table S1 and showed in the Figure 1A.

Bottom Line: Wang.For operational guidelines, the downstream populations which occupy high and peculiar haplotypes should be given prior in-situ conservation.In addition, ex-situ conservation and reintroduction measures for decades of generations are supplemented for improving the population size and genetic diversity of the endemic and endangered species.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, China ; Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, China ; University of Chinese Academy of Sciences Beijing, China.

ABSTRACT
Delimitating species boundaries could be of critical importance when evaluating the species' evolving process and providing guidelines for conservation genetics. Here, species delimitation was carried out on three endemic and endangered Cycas species with resembling morphology and overlapped distribution range along the Red River (Yuanjiang) in China: Cycas diananensis Z. T. Guan et G. D. Tao, Cycas parvula S. L. Yang and Cycas multiovula D. Y. Wang. A total of 137 individuals from 15 populations were genotyped by using three chloroplastic (psbA-trnH, atpI-atpH, and trnL-rps4) and two single copy nuclear (RPB1 and SmHP) DNA sequences. Basing on the carefully morphological comparison and cladistic haplotype aggregation (CHA) analysis, we propose all the populations as one species, with the rest two incorporated into C. diannanensis. Genetic diversity and structure analysis of the conflated C. diannanensis revealed this species possessed a relative lower genetic diversity than estimates of other Cycas species. The higher genetic diversity among populations and relative lower genetic diversity within populations, as well as obvious genetic differentiation among populations inferred from chloroplastic DNA (cpDNA) suggested a recent genetic loss within this protected species. Additionally, a clear genetic structure of C. diannanensis corresponding with geography was detected based on cpDNA, dividing its population ranges into "Yuanjiang-Nanhun" basin and "Ejia-Jiepai" basin groups. Demographical history analyses based on combined cpDNA and one nuclear DNA (nDNA) SmHP both showed the population size of C. diannanensis began to decrease in Quaternary glaciation with no subsequent expansion, while another nDNA RPB1 revealed a more recent sudden expansion after long-term population size contraction, suggesting its probable bottleneck events in history. Our findings offer grounded views for clarifying species boundaries of C. diannanensis when determining the conservation objectives. For operational guidelines, the downstream populations which occupy high and peculiar haplotypes should be given prior in-situ conservation. In addition, ex-situ conservation and reintroduction measures for decades of generations are supplemented for improving the population size and genetic diversity of the endemic and endangered species.

No MeSH data available.


Related in: MedlinePlus