Limits...
Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus

Signaling pathways associated with fertility-related proteins in boar spermatozoa.The schematic was established using Pathway Studio 9.0 following a database search in PubMed. The red-highlighted protein was highly expressed in large litter size spermatozoa, while the blue-highlighted proteins were highly expressed in small litter size spermatozoa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4562270&req=5

f4: Signaling pathways associated with fertility-related proteins in boar spermatozoa.The schematic was established using Pathway Studio 9.0 following a database search in PubMed. The red-highlighted protein was highly expressed in large litter size spermatozoa, while the blue-highlighted proteins were highly expressed in small litter size spermatozoa.

Mentions: Signaling pathways were identified using Pathway Studio to understand the signaling and metabolic pathways associated with differentially expressed protein markers. Two significant networks, metabolic and receptor-signaling pathways, were identified (P < 0.05, Table 2). Glutathione metabolism was the significant pathway associated with GPx4 and GSTM3 (P < 0.05, Table 2). In addition, the vasopressin R2/STAT signaling pathway was associated with AVPR2. A schematic image was created to determine both physiological function and interactions of the proteins that were expressed differentially in the small and large litter size spermatozoa. The differentially expressed proteins were found to interact with each other or other proteins, and were also related to particular protein kinases, ligands, transcription factors, receptors, cell processes, diseases, functional classes, and small molecules (Fig. 4). At least 14 proteins were implicated among the 20 identified proteins (Fig. 4).


Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Signaling pathways associated with fertility-related proteins in boar spermatozoa.The schematic was established using Pathway Studio 9.0 following a database search in PubMed. The red-highlighted protein was highly expressed in large litter size spermatozoa, while the blue-highlighted proteins were highly expressed in small litter size spermatozoa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562270&req=5

f4: Signaling pathways associated with fertility-related proteins in boar spermatozoa.The schematic was established using Pathway Studio 9.0 following a database search in PubMed. The red-highlighted protein was highly expressed in large litter size spermatozoa, while the blue-highlighted proteins were highly expressed in small litter size spermatozoa.
Mentions: Signaling pathways were identified using Pathway Studio to understand the signaling and metabolic pathways associated with differentially expressed protein markers. Two significant networks, metabolic and receptor-signaling pathways, were identified (P < 0.05, Table 2). Glutathione metabolism was the significant pathway associated with GPx4 and GSTM3 (P < 0.05, Table 2). In addition, the vasopressin R2/STAT signaling pathway was associated with AVPR2. A schematic image was created to determine both physiological function and interactions of the proteins that were expressed differentially in the small and large litter size spermatozoa. The differentially expressed proteins were found to interact with each other or other proteins, and were also related to particular protein kinases, ligands, transcription factors, receptors, cell processes, diseases, functional classes, and small molecules (Fig. 4). At least 14 proteins were implicated among the 20 identified proteins (Fig. 4).

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus