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Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus

Expression of GSTM3, GPx4 and AVPR2 in small and large litter size spermatozoa.(A) Ratios of GPx4, GSTM3 and AVPR2 [optical density (OD × mm)/α-tubulin (OD × mm)] in small and large litter size spermatozoa. The black line indicates small litter size. Data represent mean ± SEM, n = 3. Protein expression ratios denoted with an asterisk were significantly different (*P < 0.05). (B) GPx4, GSTM3, and AVPR2 were probed with anti-GSTM3, anti-GPx4, and anti-AVPR2 antibodies. (S = Small litter size, L = Large litter size).
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f3: Expression of GSTM3, GPx4 and AVPR2 in small and large litter size spermatozoa.(A) Ratios of GPx4, GSTM3 and AVPR2 [optical density (OD × mm)/α-tubulin (OD × mm)] in small and large litter size spermatozoa. The black line indicates small litter size. Data represent mean ± SEM, n = 3. Protein expression ratios denoted with an asterisk were significantly different (*P < 0.05). (B) GPx4, GSTM3, and AVPR2 were probed with anti-GSTM3, anti-GPx4, and anti-AVPR2 antibodies. (S = Small litter size, L = Large litter size).

Mentions: Western blotting was performed to validate the 2-DE results. GPx4, glutathione S-transferase Mu3 (GSTM3), and AVPR2 were detected at positions corresponding with ~22, 27 and 40 kDa, respectively. The intensity of the GPx4, GSTM3, and AVPR2 bands was significantly greater in the small litter size samples (P < 0.05, Fig. 3).


Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Expression of GSTM3, GPx4 and AVPR2 in small and large litter size spermatozoa.(A) Ratios of GPx4, GSTM3 and AVPR2 [optical density (OD × mm)/α-tubulin (OD × mm)] in small and large litter size spermatozoa. The black line indicates small litter size. Data represent mean ± SEM, n = 3. Protein expression ratios denoted with an asterisk were significantly different (*P < 0.05). (B) GPx4, GSTM3, and AVPR2 were probed with anti-GSTM3, anti-GPx4, and anti-AVPR2 antibodies. (S = Small litter size, L = Large litter size).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562270&req=5

f3: Expression of GSTM3, GPx4 and AVPR2 in small and large litter size spermatozoa.(A) Ratios of GPx4, GSTM3 and AVPR2 [optical density (OD × mm)/α-tubulin (OD × mm)] in small and large litter size spermatozoa. The black line indicates small litter size. Data represent mean ± SEM, n = 3. Protein expression ratios denoted with an asterisk were significantly different (*P < 0.05). (B) GPx4, GSTM3, and AVPR2 were probed with anti-GSTM3, anti-GPx4, and anti-AVPR2 antibodies. (S = Small litter size, L = Large litter size).
Mentions: Western blotting was performed to validate the 2-DE results. GPx4, glutathione S-transferase Mu3 (GSTM3), and AVPR2 were detected at positions corresponding with ~22, 27 and 40 kDa, respectively. The intensity of the GPx4, GSTM3, and AVPR2 bands was significantly greater in the small litter size samples (P < 0.05, Fig. 3).

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus