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Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus

Localization of GPx4 and AVPR2 in boar spermatozoa.(A) Merged image of nucleus (DAPI, blue) and the acrosome (lectin PNA, red), and GPx4 (green). (B) Merged image of the nucleus, the acrosome, and AVPR2 (green). Images were obtained using a Nikon TS-1000 microscope and NIS Elements software (Nikon, Japan).
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f2: Localization of GPx4 and AVPR2 in boar spermatozoa.(A) Merged image of nucleus (DAPI, blue) and the acrosome (lectin PNA, red), and GPx4 (green). (B) Merged image of the nucleus, the acrosome, and AVPR2 (green). Images were obtained using a Nikon TS-1000 microscope and NIS Elements software (Nikon, Japan).

Mentions: To examine the localization of GPx4 and AVPR2 proteins in boar spermatozoa, immunofluorescence was conducted using antibodies for GPx4 and AVPR2, lectin PNA, and DAPI. GPx4 was localized to both the head and midpiece of the spermatozoa (Fig. 2A). The AVPR2 protein was expressed in the tip of the acrosomal region and in the midpiece (Fig. 2B). Lectin PNA co-stained in the acrosome with GPx4 and AVPR2, respectively.


Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.

Kwon WS, Oh SA, Kim YJ, Rahman MS, Park YJ, Pang MG - Sci Rep (2015)

Localization of GPx4 and AVPR2 in boar spermatozoa.(A) Merged image of nucleus (DAPI, blue) and the acrosome (lectin PNA, red), and GPx4 (green). (B) Merged image of the nucleus, the acrosome, and AVPR2 (green). Images were obtained using a Nikon TS-1000 microscope and NIS Elements software (Nikon, Japan).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562270&req=5

f2: Localization of GPx4 and AVPR2 in boar spermatozoa.(A) Merged image of nucleus (DAPI, blue) and the acrosome (lectin PNA, red), and GPx4 (green). (B) Merged image of the nucleus, the acrosome, and AVPR2 (green). Images were obtained using a Nikon TS-1000 microscope and NIS Elements software (Nikon, Japan).
Mentions: To examine the localization of GPx4 and AVPR2 proteins in boar spermatozoa, immunofluorescence was conducted using antibodies for GPx4 and AVPR2, lectin PNA, and DAPI. GPx4 was localized to both the head and midpiece of the spermatozoa (Fig. 2A). The AVPR2 protein was expressed in the tip of the acrosomal region and in the midpiece (Fig. 2B). Lectin PNA co-stained in the acrosome with GPx4 and AVPR2, respectively.

Bottom Line: Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group.We then identified signaling pathways associated with the differentially expressed protein markers.Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Korea.

ABSTRACT
The ability to predict male fertility is of paramount importance for animal breeding industries and for human reproduction. Conventional semen analysis generally provides information on the quantitative parameters of spermatozoa, but yields no information concerning its functional competence. Proteomics have identified candidates for male fertility biomarkers, but no studies have clearly identified the relationship between the proteome and sperm fertility. Therefore, we performed a proteomic analysis to investigate small and large litter size boar spermatozoa and identify proteins related to male fertility. In this study, 20 proteins showed differential expression levels in small and large litter size groups. Nineteen of these proteins exhibited decreased expression in large litter size samples and increased expression in the small litter group. Interestingly, only one protein was highly expressed in the large litter size spermatozoa. We then identified signaling pathways associated with the differentially expressed protein markers. Glutathione S-transferase Mu3 and glutathione peroxidase 4 were related to the glutathione metabolic pathway and arginine vasopressin receptor 2 was linked to vasopressin R2/STAT. In summary, this is the first study to consider negative fertility biomarkers, and the identified proteins could potentially be used as biomarkers for the detection of inferior male fertility.

No MeSH data available.


Related in: MedlinePlus