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Phytotoxin coronatine enhances heat tolerance via maintaining photosynthetic performance in wheat based on Electrophoresis and TOF-MS analysis.

Zhou Y, Zhang M, Li J, Li Z, Tian X, Duan L - Sci Rep (2015)

Bottom Line: We then employed dimensional gel electrophoresis technology (2-DE) and MALDI-TOF-MS to sequester and identify the sensitive proteins.We found a total of 27 coronatine sensitive proteins, 22 of which were located in the chloroplast and 6 of which were directly involved in photosynthesis.Finally, we measured levels of chlorophyll and photosynthetic performance to reveal the phenotypic effect of these proteins.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, No 2 Yuanmingyuan Xi Lu, Haidian District, Beijing 100193, China.

ABSTRACT
Coronatine (COR) is a phytotoxin produced by Pseudomonas syringae. Its structure is similar to Jasmonates, which play a number of diverse roles in plant defense. Both have the COI1 plant receptor, so coronatine can manipulate plant hormone signaling to access nutrients and counteract defense responses. In addition to the hormone system, coronatine affects plant nitrogenous metabolism and chloroplast ultrastructure. In this study, we first examined a typical nitrogen-losing phenotype, and used the polyacrylamide gel approach to demonstrate soluble total protein patterns in a time-course experiment under different temperature conditions. We then employed dimensional gel electrophoresis technology (2-DE) and MALDI-TOF-MS to sequester and identify the sensitive proteins. We found a total of 27 coronatine sensitive proteins, 22 of which were located in the chloroplast and 6 of which were directly involved in photosynthesis. Finally, we measured levels of chlorophyll and photosynthetic performance to reveal the phenotypic effect of these proteins. Taken together, these results demonstrated that coronatine enhanced heat tolerance by regulating nitrogenous metabolism and chloroplast ultrastructure to maintain photosynthetic performance and reduce yield loss under heat stress.

No MeSH data available.


Related in: MedlinePlus

Biological process analysis of proteins induced by coronatine under heat stress.Expression diagram of selected proteins that are differentially regulated by treatment Control, COR, Heat and Heat + COR. Each protein was classified with respect to its cellular component, biological process, and molecular function. Three major groups are indicated: group I, proteins belonging to photosynthesis and chloroplast organization; group II, proteins involved in positive regulation of defense; group III, proteins responsive to negative regulation of stress, phosphorylation and plant immune system. Protein expression pattern analysis was performed using Cluster 3.0.
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f3: Biological process analysis of proteins induced by coronatine under heat stress.Expression diagram of selected proteins that are differentially regulated by treatment Control, COR, Heat and Heat + COR. Each protein was classified with respect to its cellular component, biological process, and molecular function. Three major groups are indicated: group I, proteins belonging to photosynthesis and chloroplast organization; group II, proteins involved in positive regulation of defense; group III, proteins responsive to negative regulation of stress, phosphorylation and plant immune system. Protein expression pattern analysis was performed using Cluster 3.0.

Mentions: We performed time course experiment to monitor production of soluble total protein. Results showed that coronatine pretreatment could change protein expression patterns. Heat shock proteins were induced by coronatine at an early stage, and disappeared at a later stage. Heat shock proteins reached a maximum concentration at 36 hours after coronatine treatment, and 24 hours delay than control plants (Fig. 2a). Depend on the time course results, we employed dimensional gel electrophoresis technology to identify twenty seven coronatine inducible proteins. Twenty two of these proteins are located in the chloroplast, and six are directly involved in photosynthesis (Fig. 2b,c; Table 2). When heat stress occurred, most of proteins significantly decreased their expression level, while coronatine pretreatment increased the stability of these proteins under heat stress (Figs 2c and 3).


Phytotoxin coronatine enhances heat tolerance via maintaining photosynthetic performance in wheat based on Electrophoresis and TOF-MS analysis.

Zhou Y, Zhang M, Li J, Li Z, Tian X, Duan L - Sci Rep (2015)

Biological process analysis of proteins induced by coronatine under heat stress.Expression diagram of selected proteins that are differentially regulated by treatment Control, COR, Heat and Heat + COR. Each protein was classified with respect to its cellular component, biological process, and molecular function. Three major groups are indicated: group I, proteins belonging to photosynthesis and chloroplast organization; group II, proteins involved in positive regulation of defense; group III, proteins responsive to negative regulation of stress, phosphorylation and plant immune system. Protein expression pattern analysis was performed using Cluster 3.0.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562268&req=5

f3: Biological process analysis of proteins induced by coronatine under heat stress.Expression diagram of selected proteins that are differentially regulated by treatment Control, COR, Heat and Heat + COR. Each protein was classified with respect to its cellular component, biological process, and molecular function. Three major groups are indicated: group I, proteins belonging to photosynthesis and chloroplast organization; group II, proteins involved in positive regulation of defense; group III, proteins responsive to negative regulation of stress, phosphorylation and plant immune system. Protein expression pattern analysis was performed using Cluster 3.0.
Mentions: We performed time course experiment to monitor production of soluble total protein. Results showed that coronatine pretreatment could change protein expression patterns. Heat shock proteins were induced by coronatine at an early stage, and disappeared at a later stage. Heat shock proteins reached a maximum concentration at 36 hours after coronatine treatment, and 24 hours delay than control plants (Fig. 2a). Depend on the time course results, we employed dimensional gel electrophoresis technology to identify twenty seven coronatine inducible proteins. Twenty two of these proteins are located in the chloroplast, and six are directly involved in photosynthesis (Fig. 2b,c; Table 2). When heat stress occurred, most of proteins significantly decreased their expression level, while coronatine pretreatment increased the stability of these proteins under heat stress (Figs 2c and 3).

Bottom Line: We then employed dimensional gel electrophoresis technology (2-DE) and MALDI-TOF-MS to sequester and identify the sensitive proteins.We found a total of 27 coronatine sensitive proteins, 22 of which were located in the chloroplast and 6 of which were directly involved in photosynthesis.Finally, we measured levels of chlorophyll and photosynthetic performance to reveal the phenotypic effect of these proteins.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, No 2 Yuanmingyuan Xi Lu, Haidian District, Beijing 100193, China.

ABSTRACT
Coronatine (COR) is a phytotoxin produced by Pseudomonas syringae. Its structure is similar to Jasmonates, which play a number of diverse roles in plant defense. Both have the COI1 plant receptor, so coronatine can manipulate plant hormone signaling to access nutrients and counteract defense responses. In addition to the hormone system, coronatine affects plant nitrogenous metabolism and chloroplast ultrastructure. In this study, we first examined a typical nitrogen-losing phenotype, and used the polyacrylamide gel approach to demonstrate soluble total protein patterns in a time-course experiment under different temperature conditions. We then employed dimensional gel electrophoresis technology (2-DE) and MALDI-TOF-MS to sequester and identify the sensitive proteins. We found a total of 27 coronatine sensitive proteins, 22 of which were located in the chloroplast and 6 of which were directly involved in photosynthesis. Finally, we measured levels of chlorophyll and photosynthetic performance to reveal the phenotypic effect of these proteins. Taken together, these results demonstrated that coronatine enhanced heat tolerance by regulating nitrogenous metabolism and chloroplast ultrastructure to maintain photosynthetic performance and reduce yield loss under heat stress.

No MeSH data available.


Related in: MedlinePlus