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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Number and length of CGRP positive fibers.(A) Quantitative analyses of CGRP positive fibers number showed no significant differences between individual experimental groups. (B) Statistical significance was observed only in the length of CGRP positive fibers between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (*P < 0.05, **P < 0.01, ***P < 0.001).
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f9: Number and length of CGRP positive fibers.(A) Quantitative analyses of CGRP positive fibers number showed no significant differences between individual experimental groups. (B) Statistical significance was observed only in the length of CGRP positive fibers between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (*P < 0.05, **P < 0.01, ***P < 0.001).

Mentions: CGRP immunoreactivity was observed in all experimental groups (SCI+ALG+GFs, SCI+ALG, SCI+SAL) in fibres and punctuate terminals of superficial dorsal horn (Laminae I–III) and LT (LT-Lissauer’s tract) area44 located along the lateral edge of the dorsal horn and medial grey mater (Fig. 8). Moreover, depending on the experimental group, few individual CGRP positive fibres extending from Lamina III toward Laminae V (0.226 ± 0.099 mm/SCI+SAL) and VII (Figs 8 and 9) were detected. The longest CGRP+ fibres with the average of length 0.301 ± 0.103 mm were observed after administration of alginate biomaterial alone and alginate biomaterial with affinity-bound GFs to the injured spinal cord (0.301 ± 0.103 mm/SCI+ALG; 0.27 ± 0.053 mm/SCI+ALG+GFs). Sham spinal cord didn’t contain CGRP positive fibres extended into the intermedia spinal cord layers; however CGRP terminals within superficial dorsal horn were frequently observed. The differences associated with length of fibres show statistical significance (**P < 0.01, *P < 0.05) only between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (Fig. 9).


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Number and length of CGRP positive fibers.(A) Quantitative analyses of CGRP positive fibers number showed no significant differences between individual experimental groups. (B) Statistical significance was observed only in the length of CGRP positive fibers between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (*P < 0.05, **P < 0.01, ***P < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f9: Number and length of CGRP positive fibers.(A) Quantitative analyses of CGRP positive fibers number showed no significant differences between individual experimental groups. (B) Statistical significance was observed only in the length of CGRP positive fibers between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (*P < 0.05, **P < 0.01, ***P < 0.001).
Mentions: CGRP immunoreactivity was observed in all experimental groups (SCI+ALG+GFs, SCI+ALG, SCI+SAL) in fibres and punctuate terminals of superficial dorsal horn (Laminae I–III) and LT (LT-Lissauer’s tract) area44 located along the lateral edge of the dorsal horn and medial grey mater (Fig. 8). Moreover, depending on the experimental group, few individual CGRP positive fibres extending from Lamina III toward Laminae V (0.226 ± 0.099 mm/SCI+SAL) and VII (Figs 8 and 9) were detected. The longest CGRP+ fibres with the average of length 0.301 ± 0.103 mm were observed after administration of alginate biomaterial alone and alginate biomaterial with affinity-bound GFs to the injured spinal cord (0.301 ± 0.103 mm/SCI+ALG; 0.27 ± 0.053 mm/SCI+ALG+GFs). Sham spinal cord didn’t contain CGRP positive fibres extended into the intermedia spinal cord layers; however CGRP terminals within superficial dorsal horn were frequently observed. The differences associated with length of fibres show statistical significance (**P < 0.01, *P < 0.05) only between Sham and other experimental groups (SCI+SAL, SCI+ALG, SCI+ALG+GFs) (Fig. 9).

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus