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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Representative transverse sections revealing ChAT positive motoneurons located rostrally and caudally to the lesion site following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups.Significant decrease of ChAT immunohistochemical staining was observed in ventral horns caudally to the lesion site (lower panel) following SCI and saline delivery. Injected alginate supports survival of ChAT positive motoneurons. Scale bar = 500 μm.
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f6: Representative transverse sections revealing ChAT positive motoneurons located rostrally and caudally to the lesion site following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups.Significant decrease of ChAT immunohistochemical staining was observed in ventral horns caudally to the lesion site (lower panel) following SCI and saline delivery. Injected alginate supports survival of ChAT positive motoneurons. Scale bar = 500 μm.

Mentions: The average number of ChAT positive cells in the SCI+SAL, SCI+ALG and SCI+ALG+GFs groups was compared to confirm the hypothesis whether neuronal sparing has included motor neurons of ventral horns. Rostral to the lesion site, the number of spared ChAT+ neurons within the ventral horns significantly increased (*P < 0.05) following alginate biomaterial treatment (10 ± 2.1/SCI+ALG; 10.9 ± 1.7/SCI+ALG+GFs) when compared to the control saline group (7.4 ± 0.9) (Figs 5 and 6). Significant differences in sparing of motor neurons (*P < 0.05, ***P < 0.001) were also recorded among experimental groups caudal to the injury site, although the average number of positive cells had declined (6.9 ± 1.5/SCI+ALG+GFs, 5.4 ± 0.9/SCI+ALG, 2.8 ± 0.8/SCI+SAL, 11.9 ± 1.9/Sham) compared to spinal rostral part (Figs 5 and 6). Our results demonstrate that alginate biomaterial implantation resulted not only in common NeuN positive neurons sparing, but also in the specific sparing of endogenous ChAT+ motor neurons.


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Representative transverse sections revealing ChAT positive motoneurons located rostrally and caudally to the lesion site following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups.Significant decrease of ChAT immunohistochemical staining was observed in ventral horns caudally to the lesion site (lower panel) following SCI and saline delivery. Injected alginate supports survival of ChAT positive motoneurons. Scale bar = 500 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f6: Representative transverse sections revealing ChAT positive motoneurons located rostrally and caudally to the lesion site following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups.Significant decrease of ChAT immunohistochemical staining was observed in ventral horns caudally to the lesion site (lower panel) following SCI and saline delivery. Injected alginate supports survival of ChAT positive motoneurons. Scale bar = 500 μm.
Mentions: The average number of ChAT positive cells in the SCI+SAL, SCI+ALG and SCI+ALG+GFs groups was compared to confirm the hypothesis whether neuronal sparing has included motor neurons of ventral horns. Rostral to the lesion site, the number of spared ChAT+ neurons within the ventral horns significantly increased (*P < 0.05) following alginate biomaterial treatment (10 ± 2.1/SCI+ALG; 10.9 ± 1.7/SCI+ALG+GFs) when compared to the control saline group (7.4 ± 0.9) (Figs 5 and 6). Significant differences in sparing of motor neurons (*P < 0.05, ***P < 0.001) were also recorded among experimental groups caudal to the injury site, although the average number of positive cells had declined (6.9 ± 1.5/SCI+ALG+GFs, 5.4 ± 0.9/SCI+ALG, 2.8 ± 0.8/SCI+SAL, 11.9 ± 1.9/Sham) compared to spinal rostral part (Figs 5 and 6). Our results demonstrate that alginate biomaterial implantation resulted not only in common NeuN positive neurons sparing, but also in the specific sparing of endogenous ChAT+ motor neurons.

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus