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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Stereological analyses of NeuN positive cells in Laminae I–IV, Laminae IV–V and Laminae VIII-IX after SCI and treatment.Number of NeuN labeled neurons in all studied areas increased after pure and enriched alginate administration compared to saline. Among the experimental groups we observed statistical differences (***P < 0.001, **P < 0.01, *P < 0.05).
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f4: Stereological analyses of NeuN positive cells in Laminae I–IV, Laminae IV–V and Laminae VIII-IX after SCI and treatment.Number of NeuN labeled neurons in all studied areas increased after pure and enriched alginate administration compared to saline. Among the experimental groups we observed statistical differences (***P < 0.001, **P < 0.01, *P < 0.05).

Mentions: Quantification analyses of representative transverse sections were processed bilaterally in dorsal (Laminae I–IV), ventral horns (Laminae VIII–IX) and Laminae IV–V, rostrally and caudally from the lesion site (Fig. 4). The higher number of NeuN-positive cells was documented rostrally from the injury site in all studied groups SCI+ALG+GFs, SCI+ALG and SCI+SAL. The most profound neuronal loss was observed in the SCI+SAL group (Rostral/Laminae I–IV 69.5 ± 8.1; Laminae VIII–IX 13 ± 1.5; Caudal/Laminae I–IV 68.8 ± 17.7; Laminae VIII–IX11.8 ± 5.8). The delivery of ALG or ALG+GFs promoted the survival of neuronal cells, resulting in a significant increase in number of NeuN-positive cells SCI+ALG: Rostral/Laminae I–IV 103.1 ± 26.6; Laminae VIII–IX 22 ± 5.5; Caudal/Laminae I–IV 103.3 ± 16.8; Laminae VIII–IX 15.8 ± 2,7; SCI+ALG+GFs: Rostral/Laminae I–IV 110 ± 5.3; Laminae VIII–IX 27.3 ± 4.4; Caudal/Laminae I–IV 125.5 ± 4.2; Laminae VIII–IX 23.6 ± 6.4). Moreover, the number of NeuN positive cells in the SCI+ALG+GFs group closely correlated with the NeuN numbers observed in Sham group (Rostral/Laminae I–IV 122 ± 5.2; Laminae VIII–IX 23.1 ± 5; Caudal/Laminae I–IV 120.7 ± 12.7; Laminae VIII–IX24 ± 3.8) (Fig. 4). The differences in NeuN positive profiles show a statistical significance between individual experimental groups: Sham, SCI+SAL, SCI+ALG, SCI+ALG+GFs ***P < 0.001, **P < 0.01, *P < 0.05.


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Stereological analyses of NeuN positive cells in Laminae I–IV, Laminae IV–V and Laminae VIII-IX after SCI and treatment.Number of NeuN labeled neurons in all studied areas increased after pure and enriched alginate administration compared to saline. Among the experimental groups we observed statistical differences (***P < 0.001, **P < 0.01, *P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f4: Stereological analyses of NeuN positive cells in Laminae I–IV, Laminae IV–V and Laminae VIII-IX after SCI and treatment.Number of NeuN labeled neurons in all studied areas increased after pure and enriched alginate administration compared to saline. Among the experimental groups we observed statistical differences (***P < 0.001, **P < 0.01, *P < 0.05).
Mentions: Quantification analyses of representative transverse sections were processed bilaterally in dorsal (Laminae I–IV), ventral horns (Laminae VIII–IX) and Laminae IV–V, rostrally and caudally from the lesion site (Fig. 4). The higher number of NeuN-positive cells was documented rostrally from the injury site in all studied groups SCI+ALG+GFs, SCI+ALG and SCI+SAL. The most profound neuronal loss was observed in the SCI+SAL group (Rostral/Laminae I–IV 69.5 ± 8.1; Laminae VIII–IX 13 ± 1.5; Caudal/Laminae I–IV 68.8 ± 17.7; Laminae VIII–IX11.8 ± 5.8). The delivery of ALG or ALG+GFs promoted the survival of neuronal cells, resulting in a significant increase in number of NeuN-positive cells SCI+ALG: Rostral/Laminae I–IV 103.1 ± 26.6; Laminae VIII–IX 22 ± 5.5; Caudal/Laminae I–IV 103.3 ± 16.8; Laminae VIII–IX 15.8 ± 2,7; SCI+ALG+GFs: Rostral/Laminae I–IV 110 ± 5.3; Laminae VIII–IX 27.3 ± 4.4; Caudal/Laminae I–IV 125.5 ± 4.2; Laminae VIII–IX 23.6 ± 6.4). Moreover, the number of NeuN positive cells in the SCI+ALG+GFs group closely correlated with the NeuN numbers observed in Sham group (Rostral/Laminae I–IV 122 ± 5.2; Laminae VIII–IX 23.1 ± 5; Caudal/Laminae I–IV 120.7 ± 12.7; Laminae VIII–IX24 ± 3.8) (Fig. 4). The differences in NeuN positive profiles show a statistical significance between individual experimental groups: Sham, SCI+SAL, SCI+ALG, SCI+ALG+GFs ***P < 0.001, **P < 0.01, *P < 0.05.

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus