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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Morphometric analyses of cavity length (A) and size (B) in experimental groups showed significant reduction after intraspinal injection of biomaterial alginate (**P < 0.01, ***P < 0.001). Cavity size was expressed by mm in injured experimental groups (SCI+SAL, SCI+ALG and SCI+ALG+GFs) relative to Sham spinal cord without cavitations represent by zero.
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f3: Morphometric analyses of cavity length (A) and size (B) in experimental groups showed significant reduction after intraspinal injection of biomaterial alginate (**P < 0.01, ***P < 0.001). Cavity size was expressed by mm in injured experimental groups (SCI+SAL, SCI+ALG and SCI+ALG+GFs) relative to Sham spinal cord without cavitations represent by zero.

Mentions: During the first week post-injury, a severe inflammatory response occurs at the central lesion. The secondary damage processes lead to cell death and development of cavitations at the epicenter and along the rostrocaudal axis of the spinal cord40. In order to fill the cavity and create a permissive environment for regeneration, we administered the liquid form of alginate scaffold directly to the lesion cavity at 7D post injury. Histological assessment of spinal cord sections stained with Luxol Fast Blue revealed cavity area reduction in SCI+ALG+GFs and SCI+ALG groups compared to SCI+SAL at 42 day post-implantation (Fig. 3). Quantitative stereological analyses of tissue fenestration in 1.6 cm segment revealed significant (**P < 0.01, ***P < 0.001) reduction of cavitation (analysing length and area of cavity) after application of ALG+GFs (cavity length/3.3 ± 1.5 mm/area/0.56 ± 0.2 mm2) and ALG (cavity length/5.4 ± 1.2 mm/area/1.13 ± 0.2 mm2) compared to the saline treatment (cavity length/7.7 ± 1.4 mm/area/1.96 ± 0.3 mm2) (Fig. 3).


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Morphometric analyses of cavity length (A) and size (B) in experimental groups showed significant reduction after intraspinal injection of biomaterial alginate (**P < 0.01, ***P < 0.001). Cavity size was expressed by mm in injured experimental groups (SCI+SAL, SCI+ALG and SCI+ALG+GFs) relative to Sham spinal cord without cavitations represent by zero.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f3: Morphometric analyses of cavity length (A) and size (B) in experimental groups showed significant reduction after intraspinal injection of biomaterial alginate (**P < 0.01, ***P < 0.001). Cavity size was expressed by mm in injured experimental groups (SCI+SAL, SCI+ALG and SCI+ALG+GFs) relative to Sham spinal cord without cavitations represent by zero.
Mentions: During the first week post-injury, a severe inflammatory response occurs at the central lesion. The secondary damage processes lead to cell death and development of cavitations at the epicenter and along the rostrocaudal axis of the spinal cord40. In order to fill the cavity and create a permissive environment for regeneration, we administered the liquid form of alginate scaffold directly to the lesion cavity at 7D post injury. Histological assessment of spinal cord sections stained with Luxol Fast Blue revealed cavity area reduction in SCI+ALG+GFs and SCI+ALG groups compared to SCI+SAL at 42 day post-implantation (Fig. 3). Quantitative stereological analyses of tissue fenestration in 1.6 cm segment revealed significant (**P < 0.01, ***P < 0.001) reduction of cavitation (analysing length and area of cavity) after application of ALG+GFs (cavity length/3.3 ± 1.5 mm/area/0.56 ± 0.2 mm2) and ALG (cavity length/5.4 ± 1.2 mm/area/1.13 ± 0.2 mm2) compared to the saline treatment (cavity length/7.7 ± 1.4 mm/area/1.96 ± 0.3 mm2) (Fig. 3).

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus