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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, BlaΕ‘ko J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Functional recovery of hindlimb motor (A) and sensory functions (B) following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups. (A) *P < 0.05 indicates significant differences among the experimental groups.
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f2: Functional recovery of hindlimb motor (A) and sensory functions (B) following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups. (A) *P < 0.05 indicates significant differences among the experimental groups.

Mentions: During the initial days post-injury, the compression caused hindlimb paralysis with slight movement in one or two joints in all experimental groups. On following days, the animals in the SCI+ALG and SCI+ALG+GFs biomaterial treatment groups showed a similar gradual recovery of hindlimb locomotion up to 21D after SCI; greater than the recovery in the SCI+SAL group, where only limited recovery of motor function was noted. The significant locomotor improvement (*p < 0.05) between SCI+SAL and SCI+ALG/SCI+ALG+GFs was detected at 12 and 14 days post-injury with the final BBB scores 7.3 ± 2.5/12D, 8.4 ± 2.7/14D (SCI+ALG+GFs), 6.9 ± 2.1/12D, 7.8 ± 2.9/14D (SCI+ALG) and 4 ± 2.4/12D, 4.5 ± 2.714D (SCI+SAL) (Fig. 2A). The BBB score of hindlimb motor function gradually increased in all experimental groups with the survival (21, 28, 35, 42 and 49 days post-surgery), however the highest scores were observed in SCI+ALG+GFs (10.8 ± 3, 12.9 ± 3.6, 13.1 ± 1.2, 13.8 ± 3.1, 14.0 ± 3), which closely correlated with SCI+ALG (10.5 ± 2.5, 11.5 ± 1.4, 12.4 ± 0.9, 12.7 ± 0.9, 12.9 ± 1.9) but the increase was less prominent. The SCI+SAL group following initial gradual motor function improvement at 8 to 28 days, showed only limited recovery during further survival (7.4 ± 3.6/21D, 9.5 ± 3.6/28D, 10 ± 3.1/35D, 10.6 ± 2.9/42D, 11.4 ± 3/49D) (Fig. 2A).


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, BlaΕ‘ko J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Functional recovery of hindlimb motor (A) and sensory functions (B) following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups. (A) *P < 0.05 indicates significant differences among the experimental groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f2: Functional recovery of hindlimb motor (A) and sensory functions (B) following SCI in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs experimental groups. (A) *P < 0.05 indicates significant differences among the experimental groups.
Mentions: During the initial days post-injury, the compression caused hindlimb paralysis with slight movement in one or two joints in all experimental groups. On following days, the animals in the SCI+ALG and SCI+ALG+GFs biomaterial treatment groups showed a similar gradual recovery of hindlimb locomotion up to 21D after SCI; greater than the recovery in the SCI+SAL group, where only limited recovery of motor function was noted. The significant locomotor improvement (*p < 0.05) between SCI+SAL and SCI+ALG/SCI+ALG+GFs was detected at 12 and 14 days post-injury with the final BBB scores 7.3 ± 2.5/12D, 8.4 ± 2.7/14D (SCI+ALG+GFs), 6.9 ± 2.1/12D, 7.8 ± 2.9/14D (SCI+ALG) and 4 ± 2.4/12D, 4.5 ± 2.714D (SCI+SAL) (Fig. 2A). The BBB score of hindlimb motor function gradually increased in all experimental groups with the survival (21, 28, 35, 42 and 49 days post-surgery), however the highest scores were observed in SCI+ALG+GFs (10.8 ± 3, 12.9 ± 3.6, 13.1 ± 1.2, 13.8 ± 3.1, 14.0 ± 3), which closely correlated with SCI+ALG (10.5 ± 2.5, 11.5 ± 1.4, 12.4 ± 0.9, 12.7 ± 0.9, 12.9 ± 1.9) but the increase was less prominent. The SCI+SAL group following initial gradual motor function improvement at 8 to 28 days, showed only limited recovery during further survival (7.4 ± 3.6/21D, 9.5 ± 3.6/28D, 10 ± 3.1/35D, 10.6 ± 2.9/42D, 11.4 ± 3/49D) (Fig. 2A).

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus