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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

Representative transverse sections of Iba1 expression from caudal and rostral segments of spinal cord.Sections illustrate changes in activation and cell morphology after saline and alginate treatment. Baseline expression of non-active microglia in gray and white matter was observed in Sham animals. Strong activation of microglia characterized by enlarged round-shaped perikaryon with truncated, dick and ramified processes was detected in SCI+SAL group. Alginate treatment (SCI+ALG and SCI+ALG+GFs groups) inhibited activation of Iba1 positive cells. Scale bars = 500 μm, 10 μm (higher magnification of boxed area).
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f12: Representative transverse sections of Iba1 expression from caudal and rostral segments of spinal cord.Sections illustrate changes in activation and cell morphology after saline and alginate treatment. Baseline expression of non-active microglia in gray and white matter was observed in Sham animals. Strong activation of microglia characterized by enlarged round-shaped perikaryon with truncated, dick and ramified processes was detected in SCI+SAL group. Alginate treatment (SCI+ALG and SCI+ALG+GFs groups) inhibited activation of Iba1 positive cells. Scale bars = 500 μm, 10 μm (higher magnification of boxed area).

Mentions: In order to monitor the immune response of host tissue, particularly the presence of microglia cells after alginate biomaterial treatment, Iba1 immunoreactivity in lesion site and also in the adjacent segments located 0.8 cm rostrally/caudally to the epicenter, were used. Enhancement of the microglia responsiveness and subsequent density was observed mainly after injury with injection of saline (SCI+SAL: Ros/17 ± 3, Ros-Centre/23.3 ± 3.4, Centre-Caud/22.3 ± 4.4, Caud/21.3 ± 4.9), while the decreased tendency of Iba1 expression was seen after treatment with alginate biomaterial (SCI+ALG+GFs: Ros/14.8 ± 2.1, Ros-Centre/17.3 ± 4.3, Centre-Caud/18.1 ± 3, Caud/17.4 ± 2.9; SCI+ALG: Ros/16.4 ± 2.5, Ros-Centre/20.5 ± 3.2, Centre-Caud/17.6 ± 4.2, Caud/14.2 ± 4.3) (Fig. 12, Supplementary Figure 5). Positivity of Iba1 expression in Sham animals revealed basaline levels (Ros/9.4 ± 1.5, Ros-Centre/9.3 ± 0.8, Centre-Caud/9.3 ± 1.6, Caud/9.8 ± 1.6). Among experimental groups and individual parts of spinal cord significant differences were detected (***P < 0.001, **P < 0.01, *P < 0.05, Supplementary Figure 5).


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Representative transverse sections of Iba1 expression from caudal and rostral segments of spinal cord.Sections illustrate changes in activation and cell morphology after saline and alginate treatment. Baseline expression of non-active microglia in gray and white matter was observed in Sham animals. Strong activation of microglia characterized by enlarged round-shaped perikaryon with truncated, dick and ramified processes was detected in SCI+SAL group. Alginate treatment (SCI+ALG and SCI+ALG+GFs groups) inhibited activation of Iba1 positive cells. Scale bars = 500 μm, 10 μm (higher magnification of boxed area).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f12: Representative transverse sections of Iba1 expression from caudal and rostral segments of spinal cord.Sections illustrate changes in activation and cell morphology after saline and alginate treatment. Baseline expression of non-active microglia in gray and white matter was observed in Sham animals. Strong activation of microglia characterized by enlarged round-shaped perikaryon with truncated, dick and ramified processes was detected in SCI+SAL group. Alginate treatment (SCI+ALG and SCI+ALG+GFs groups) inhibited activation of Iba1 positive cells. Scale bars = 500 μm, 10 μm (higher magnification of boxed area).
Mentions: In order to monitor the immune response of host tissue, particularly the presence of microglia cells after alginate biomaterial treatment, Iba1 immunoreactivity in lesion site and also in the adjacent segments located 0.8 cm rostrally/caudally to the epicenter, were used. Enhancement of the microglia responsiveness and subsequent density was observed mainly after injury with injection of saline (SCI+SAL: Ros/17 ± 3, Ros-Centre/23.3 ± 3.4, Centre-Caud/22.3 ± 4.4, Caud/21.3 ± 4.9), while the decreased tendency of Iba1 expression was seen after treatment with alginate biomaterial (SCI+ALG+GFs: Ros/14.8 ± 2.1, Ros-Centre/17.3 ± 4.3, Centre-Caud/18.1 ± 3, Caud/17.4 ± 2.9; SCI+ALG: Ros/16.4 ± 2.5, Ros-Centre/20.5 ± 3.2, Centre-Caud/17.6 ± 4.2, Caud/14.2 ± 4.3) (Fig. 12, Supplementary Figure 5). Positivity of Iba1 expression in Sham animals revealed basaline levels (Ros/9.4 ± 1.5, Ros-Centre/9.3 ± 0.8, Centre-Caud/9.3 ± 1.6, Caud/9.8 ± 1.6). Among experimental groups and individual parts of spinal cord significant differences were detected (***P < 0.001, **P < 0.01, *P < 0.05, Supplementary Figure 5).

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus