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Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus

BDA labeling in the representative sagittal sections in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs groups 49D post injury.Representative pictures and corresponding details (boxed areas) illustrate growth potential of CST fibers after SCI and individual treatments. Increased positivity of BDA was seen after alginate administration (SCI+ALG and SCI+ALG+GFs groups) where CST axons were growing around the lesion site towards disconnected caudal segment. Scale bar = 500 μm.
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f10: BDA labeling in the representative sagittal sections in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs groups 49D post injury.Representative pictures and corresponding details (boxed areas) illustrate growth potential of CST fibers after SCI and individual treatments. Increased positivity of BDA was seen after alginate administration (SCI+ALG and SCI+ALG+GFs groups) where CST axons were growing around the lesion site towards disconnected caudal segment. Scale bar = 500 μm.

Mentions: BDA delivery to the sensorimotor cortex served to label descending CST axons of spinal cord. In sham animals, BDA-labelled CST axons were detected along the entire length of sagittal sections (16 mm) of the spinal cord; more specifically in the ventral part of the dorsal column, where stripe of organized BDA positive axons occurred (16 mm ± 0) (Figs 10 and 11). After spinal cord injury, CST axons appeared disorganized, ended above the lesion site and many cut BDA axons formed terminal structures like buttons. Re-growth of CST fibres into denervated areas of spinal cord was monitored following alginate administration. Moreover, the alginate biomaterial alone and with affinity-bound EGF/bFGF promoted increased re-growth of few BDA positive fibres through the central lesion with occasional innervations below the lesion site (2.9 mm ± 0.7 from a total 16 mm length of section) compared to saline treatment (0.6 mm ± 0.1) (Figs 10 and 11) (*P < 0.05).


Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair.

Grulova I, Slovinska L, Blaško J, Devaux S, Wisztorski M, Salzet M, Fournier I, Kryukov O, Cohen S, Cizkova D - Sci Rep (2015)

BDA labeling in the representative sagittal sections in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs groups 49D post injury.Representative pictures and corresponding details (boxed areas) illustrate growth potential of CST fibers after SCI and individual treatments. Increased positivity of BDA was seen after alginate administration (SCI+ALG and SCI+ALG+GFs groups) where CST axons were growing around the lesion site towards disconnected caudal segment. Scale bar = 500 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562265&req=5

f10: BDA labeling in the representative sagittal sections in Sham, SCI+SAL, SCI+ALG and SCI+ALG+GFs groups 49D post injury.Representative pictures and corresponding details (boxed areas) illustrate growth potential of CST fibers after SCI and individual treatments. Increased positivity of BDA was seen after alginate administration (SCI+ALG and SCI+ALG+GFs groups) where CST axons were growing around the lesion site towards disconnected caudal segment. Scale bar = 500 μm.
Mentions: BDA delivery to the sensorimotor cortex served to label descending CST axons of spinal cord. In sham animals, BDA-labelled CST axons were detected along the entire length of sagittal sections (16 mm) of the spinal cord; more specifically in the ventral part of the dorsal column, where stripe of organized BDA positive axons occurred (16 mm ± 0) (Figs 10 and 11). After spinal cord injury, CST axons appeared disorganized, ended above the lesion site and many cut BDA axons formed terminal structures like buttons. Re-growth of CST fibres into denervated areas of spinal cord was monitored following alginate administration. Moreover, the alginate biomaterial alone and with affinity-bound EGF/bFGF promoted increased re-growth of few BDA positive fibres through the central lesion with occasional innervations below the lesion site (2.9 mm ± 0.7 from a total 16 mm length of section) compared to saline treatment (0.6 mm ± 0.1) (Figs 10 and 11) (*P < 0.05).

Bottom Line: In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion.Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed.Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neurobiology, Center of Excellence for Brain Research, Department of Regenerative Medicine and Stem Cell Therapy, Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Kosice, Slovakia.

ABSTRACT
Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffold with affinity-bound growth factors (ALG+GFs), compared to SCI animals without biomaterial treatment.

No MeSH data available.


Related in: MedlinePlus