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Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus

Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Ch.Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67 and E. coli K12 (GenBank ID: 513473511, 690276415, 282947233, 62178570, 556503834, respectively).
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fig-9: Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Ch.Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67 and E. coli K12 (GenBank ID: 513473511, 690276415, 282947233, 62178570, 556503834, respectively).

Mentions: Orphan LuxR is known to be present in E. coli and Salmonella which allows response to exogenous AHLs (Ahmer, 2004). The function of the orphan LuxR in C. neteri SSMD04 is not known. However, genome comparison of C. neteri SSMD04 and M006, C. davisae DSM4568, C. rodentium ICC168, S. enterica subsp. enterica serovar Choleraesuis str. SC-B67, and E. coli K12 shows a considerable degree of conservation (Fig. 9). This is especially true for the 3 genomes of the genus Cedecea, despite the absence of canonical luxI/R pair in C. davisae DSM4568 genome.


Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Ch.Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67 and E. coli K12 (GenBank ID: 513473511, 690276415, 282947233, 62178570, 556503834, respectively).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562240&req=5

fig-9: Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Ch.Organization of C. neteri SSMD04 orphan luxR and its flanking genes in comparison with other selected species C. davisae DSM4568, C. neteri M006, C. rodentium ICC168, Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67 and E. coli K12 (GenBank ID: 513473511, 690276415, 282947233, 62178570, 556503834, respectively).
Mentions: Orphan LuxR is known to be present in E. coli and Salmonella which allows response to exogenous AHLs (Ahmer, 2004). The function of the orphan LuxR in C. neteri SSMD04 is not known. However, genome comparison of C. neteri SSMD04 and M006, C. davisae DSM4568, C. rodentium ICC168, S. enterica subsp. enterica serovar Choleraesuis str. SC-B67, and E. coli K12 shows a considerable degree of conservation (Fig. 9). This is especially true for the 3 genomes of the genus Cedecea, despite the absence of canonical luxI/R pair in C. davisae DSM4568 genome.

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus