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Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus

Multiple sequence alignment of CneR, C. neteri SSMD04 orphan LuxR (Orphan) with five other canonical QS LuxR-type proteins.TraR, CroR, LuxR, LasR, and RhlR (GenBank ID: 282950058, 59482356, 299361, 541657, 1117981, 740696391, 689266442, respectively) are LuxR-type proteins involved in quorum sensing from A. tumefaciens, C. rodentium, Vibrio fischeri, Pseudomonas aeruginosa (LasR and RhlR), respectively. Identical residues are denoted by a vertical filled bar, and conserved residues are denoted by unfilled box. TraR residue numbering is shown above the alignment as reference.
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fig-8: Multiple sequence alignment of CneR, C. neteri SSMD04 orphan LuxR (Orphan) with five other canonical QS LuxR-type proteins.TraR, CroR, LuxR, LasR, and RhlR (GenBank ID: 282950058, 59482356, 299361, 541657, 1117981, 740696391, 689266442, respectively) are LuxR-type proteins involved in quorum sensing from A. tumefaciens, C. rodentium, Vibrio fischeri, Pseudomonas aeruginosa (LasR and RhlR), respectively. Identical residues are denoted by a vertical filled bar, and conserved residues are denoted by unfilled box. TraR residue numbering is shown above the alignment as reference.

Mentions: Apart from that, a sequence potentially encoding an orphan luxR type receptor (723 bp) was also found within the genome. This luxR homologue shares 69% sequence homology to luxR homologue of Enterobacter asburiae L1. Multiple sequence alignment of this orphan LuxR, CneR, and other canonical LuxR-type proteins (Fig. 8) reveals the presence of conserved sites, residues 57, 61, 70, 71, 85, 113, 178, 182, 188 (TraR sequence numbering used as a reference), which are present in at least 95% of LuxR-type proteins (Whitehead et al., 2001; Zhang et al., 2002).


Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Multiple sequence alignment of CneR, C. neteri SSMD04 orphan LuxR (Orphan) with five other canonical QS LuxR-type proteins.TraR, CroR, LuxR, LasR, and RhlR (GenBank ID: 282950058, 59482356, 299361, 541657, 1117981, 740696391, 689266442, respectively) are LuxR-type proteins involved in quorum sensing from A. tumefaciens, C. rodentium, Vibrio fischeri, Pseudomonas aeruginosa (LasR and RhlR), respectively. Identical residues are denoted by a vertical filled bar, and conserved residues are denoted by unfilled box. TraR residue numbering is shown above the alignment as reference.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562240&req=5

fig-8: Multiple sequence alignment of CneR, C. neteri SSMD04 orphan LuxR (Orphan) with five other canonical QS LuxR-type proteins.TraR, CroR, LuxR, LasR, and RhlR (GenBank ID: 282950058, 59482356, 299361, 541657, 1117981, 740696391, 689266442, respectively) are LuxR-type proteins involved in quorum sensing from A. tumefaciens, C. rodentium, Vibrio fischeri, Pseudomonas aeruginosa (LasR and RhlR), respectively. Identical residues are denoted by a vertical filled bar, and conserved residues are denoted by unfilled box. TraR residue numbering is shown above the alignment as reference.
Mentions: Apart from that, a sequence potentially encoding an orphan luxR type receptor (723 bp) was also found within the genome. This luxR homologue shares 69% sequence homology to luxR homologue of Enterobacter asburiae L1. Multiple sequence alignment of this orphan LuxR, CneR, and other canonical LuxR-type proteins (Fig. 8) reveals the presence of conserved sites, residues 57, 61, 70, 71, 85, 113, 178, 182, 188 (TraR sequence numbering used as a reference), which are present in at least 95% of LuxR-type proteins (Whitehead et al., 2001; Zhang et al., 2002).

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus