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Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree showing the position of C. neteri SSMD04 (green squares) relative to other Cedecea spp.The maximum likelihood tree was inferred from 1,297 aligned positions of the 16S rDNA sequences using Hasegawa-Kishino-Yano substitution model. Boostrap values are represented at the nodes. The scale denotes the number of substitutions per nucleotide position. Serratia marcescens strain HokM was used as outgroup.
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fig-1: Phylogenetic tree showing the position of C. neteri SSMD04 (green squares) relative to other Cedecea spp.The maximum likelihood tree was inferred from 1,297 aligned positions of the 16S rDNA sequences using Hasegawa-Kishino-Yano substitution model. Boostrap values are represented at the nodes. The scale denotes the number of substitutions per nucleotide position. Serratia marcescens strain HokM was used as outgroup.

Mentions: Seven 16S rRNA gene sequences were found in C. neteri SSMD04 genome, of which 6 were identical and the other has 2 SNPs. Both variants were included in phylogenetic analysis with other sequences of Cedecea spp. available in GenBank. As can be seen, 16S rRNA gene sequences of C. neteri SSMD04 and other C. neteri strains appeared in a monophyletic clade (Fig. 1).


Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor.

Tan KH, Tan JY, Yin WF, Chan KG - PeerJ (2015)

Phylogenetic tree showing the position of C. neteri SSMD04 (green squares) relative to other Cedecea spp.The maximum likelihood tree was inferred from 1,297 aligned positions of the 16S rDNA sequences using Hasegawa-Kishino-Yano substitution model. Boostrap values are represented at the nodes. The scale denotes the number of substitutions per nucleotide position. Serratia marcescens strain HokM was used as outgroup.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562240&req=5

fig-1: Phylogenetic tree showing the position of C. neteri SSMD04 (green squares) relative to other Cedecea spp.The maximum likelihood tree was inferred from 1,297 aligned positions of the 16S rDNA sequences using Hasegawa-Kishino-Yano substitution model. Boostrap values are represented at the nodes. The scale denotes the number of substitutions per nucleotide position. Serratia marcescens strain HokM was used as outgroup.
Mentions: Seven 16S rRNA gene sequences were found in C. neteri SSMD04 genome, of which 6 were identical and the other has 2 SNPs. Both variants were included in phylogenetic analysis with other sequences of Cedecea spp. available in GenBank. As can be seen, 16S rRNA gene sequences of C. neteri SSMD04 and other C. neteri strains appeared in a monophyletic clade (Fig. 1).

Bottom Line: The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04.Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR.In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.

ABSTRACT
Cedecea neteri is a very rare human pathogen. We have isolated a strain of C. neteri SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we have demonstrated the presence of short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in C. neteri SSMD04. Triple quadrupole LC/MS analysis revealed that C. neteri SSMD04 produced short chain N-butyryl-homoserine lactone (C4-HSL). With the available genome information of C. neteri SSMD04, we went on to analyse and identified a pair of luxI/R homologues in this genome that share the highest similarity with croI/R homologues from Citrobacter rodentium. The AHL synthase, which we named cneI(636 bp), was found in the genome sequences of C. neteri SSMD04. At a distance of 8bp from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. Analysis of this protein amino acid sequence reveals two signature domains, the autoinducer-binding domain and the C-terminal effector which is typical characteristic of luxR. In addition, we found that this genome harboured an orphan luxR that is most closely related to easR in Enterobacter asburiae. To our knowledge, this is the first report on the AHL production activity in C. neteri, and the discovery of its luxI/R homologues, the orphan receptor and its whole genome sequence.

No MeSH data available.


Related in: MedlinePlus