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Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus

Olfactory orange odor choices for a population of ten inbred lines and individual lines.(A) Overall proportion of orange odor choices for a population of ten inbred lines. (B) Orange odor choices after exposure to Orange first Apple second (O/A) and to Apple first, Orange second (A/O) in each of ten inbred lines.
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fig-4: Olfactory orange odor choices for a population of ten inbred lines and individual lines.(A) Overall proportion of orange odor choices for a population of ten inbred lines. (B) Orange odor choices after exposure to Orange first Apple second (O/A) and to Apple first, Orange second (A/O) in each of ten inbred lines.

Mentions: In the olfactory preference assay, the overall distribution of the orange odor choices was significantly different from the normal distribution (Shapiro–Wilk normality test: W = 0.98, p = 0.03) and we analyzed the data using non-parametric tests (Wilcoxon signed-rank test and Kruskal–Wallis test). Overall, the group of ten responsive lines showed an olfactory preference for the orange odor (mean = 0.56; V = 7862, p < 0.001; Fig. 4A). We did not observe significant differences across lines (Kruskal–Wallis Chi squared9 = 14.14, p = 0.12; see Fig. 4B) and in the effect of the order of presentation (A/O vs. O/A) (Kruskal–Wallis Chi squared9 = 1.48, p = 0.22).


Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Olfactory orange odor choices for a population of ten inbred lines and individual lines.(A) Overall proportion of orange odor choices for a population of ten inbred lines. (B) Orange odor choices after exposure to Orange first Apple second (O/A) and to Apple first, Orange second (A/O) in each of ten inbred lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562235&req=5

fig-4: Olfactory orange odor choices for a population of ten inbred lines and individual lines.(A) Overall proportion of orange odor choices for a population of ten inbred lines. (B) Orange odor choices after exposure to Orange first Apple second (O/A) and to Apple first, Orange second (A/O) in each of ten inbred lines.
Mentions: In the olfactory preference assay, the overall distribution of the orange odor choices was significantly different from the normal distribution (Shapiro–Wilk normality test: W = 0.98, p = 0.03) and we analyzed the data using non-parametric tests (Wilcoxon signed-rank test and Kruskal–Wallis test). Overall, the group of ten responsive lines showed an olfactory preference for the orange odor (mean = 0.56; V = 7862, p < 0.001; Fig. 4A). We did not observe significant differences across lines (Kruskal–Wallis Chi squared9 = 14.14, p = 0.12; see Fig. 4B) and in the effect of the order of presentation (A/O vs. O/A) (Kruskal–Wallis Chi squared9 = 1.48, p = 0.22).

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus