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Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus

Orange choices after conditioning and learning score.(A) Proportion of orange choices of flies conditioned with Orange aversive/Apple palatable (O-/A, yellow) and Apple aversive/Orange palatable (A-/O, orange). (B) Learning score: difference in the proportion of orange odor choices between flies conditioned A-/O (Apple aversive, Orange palatable) and O-/A (Orange aversive, Apple palatable).
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fig-3: Orange choices after conditioning and learning score.(A) Proportion of orange choices of flies conditioned with Orange aversive/Apple palatable (O-/A, yellow) and Apple aversive/Orange palatable (A-/O, orange). (B) Learning score: difference in the proportion of orange odor choices between flies conditioned A-/O (Apple aversive, Orange palatable) and O-/A (Orange aversive, Apple palatable).

Mentions: In the conditioning experiment, the overall population showed a preference for the orange odor (mean = 0.59, t57 = 3.95, p < 0.001). Flies that previously experienced Apple as aversive/Orange as palatable (A-/O) were more likely to choose orange than flies exposed to the opposite contingency (O-/A) (t56 = 2.24, p = 0.029; Fig. 3A). The population showed a significant learning score (t28 = 2.88, p = 0.007; Fig. 3B).


Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Orange choices after conditioning and learning score.(A) Proportion of orange choices of flies conditioned with Orange aversive/Apple palatable (O-/A, yellow) and Apple aversive/Orange palatable (A-/O, orange). (B) Learning score: difference in the proportion of orange odor choices between flies conditioned A-/O (Apple aversive, Orange palatable) and O-/A (Orange aversive, Apple palatable).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562235&req=5

fig-3: Orange choices after conditioning and learning score.(A) Proportion of orange choices of flies conditioned with Orange aversive/Apple palatable (O-/A, yellow) and Apple aversive/Orange palatable (A-/O, orange). (B) Learning score: difference in the proportion of orange odor choices between flies conditioned A-/O (Apple aversive, Orange palatable) and O-/A (Orange aversive, Apple palatable).
Mentions: In the conditioning experiment, the overall population showed a preference for the orange odor (mean = 0.59, t57 = 3.95, p < 0.001). Flies that previously experienced Apple as aversive/Orange as palatable (A-/O) were more likely to choose orange than flies exposed to the opposite contingency (O-/A) (t56 = 2.24, p = 0.029; Fig. 3A). The population showed a significant learning score (t28 = 2.88, p = 0.007; Fig. 3B).

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus