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Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus

Orange odor choices and order effect score.(A) Proportion of orange choices of flies exposed to Orange as first, Apple as second stimulus (O/A), and to Apple as first, Orange as second stimulus (A/O). (B) Order effect score (difference in orange odor choices between flies exposed to A/O and O/A).
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fig-2: Orange odor choices and order effect score.(A) Proportion of orange choices of flies exposed to Orange as first, Apple as second stimulus (O/A), and to Apple as first, Orange as second stimulus (A/O). (B) Order effect score (difference in orange odor choices between flies exposed to A/O and O/A).

Mentions: Before testing flies, we exposed them to both odors/flavors: in half trials flies were exposed first to orange then to apple (O/A), in half trials first to apple then to orange (A/O). We have derived the order effect score o to investigate the effect of the order in which the orange/apple stimuli had been presented. We observed a significant order effect score (t13 = 3.09, p = 0.009; Fig. 2B), indicating that A/O flies (flies first exposed to Apple, then to Orange) had a significantly higher preference for orange odor than O/A flies (flies first exposed to Orange, then to Apple). A post-hoc t-test on A/O and O/A flies vs. the chance level (0.5) revealed that only A/O flies had a significant preference for the orange odor: for A/O flies t13 = 3.18, p = 0.007; for O/A flies t13 = − 0.242, p = 0.81.


Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Orange odor choices and order effect score.(A) Proportion of orange choices of flies exposed to Orange as first, Apple as second stimulus (O/A), and to Apple as first, Orange as second stimulus (A/O). (B) Order effect score (difference in orange odor choices between flies exposed to A/O and O/A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562235&req=5

fig-2: Orange odor choices and order effect score.(A) Proportion of orange choices of flies exposed to Orange as first, Apple as second stimulus (O/A), and to Apple as first, Orange as second stimulus (A/O). (B) Order effect score (difference in orange odor choices between flies exposed to A/O and O/A).
Mentions: Before testing flies, we exposed them to both odors/flavors: in half trials flies were exposed first to orange then to apple (O/A), in half trials first to apple then to orange (A/O). We have derived the order effect score o to investigate the effect of the order in which the orange/apple stimuli had been presented. We observed a significant order effect score (t13 = 3.09, p = 0.009; Fig. 2B), indicating that A/O flies (flies first exposed to Apple, then to Orange) had a significantly higher preference for orange odor than O/A flies (flies first exposed to Orange, then to Apple). A post-hoc t-test on A/O and O/A flies vs. the chance level (0.5) revealed that only A/O flies had a significant preference for the orange odor: for A/O flies t13 = 3.18, p = 0.007; for O/A flies t13 = − 0.242, p = 0.81.

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus