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Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus

T-maze apparatus and experimental paradigm.(A) During Exposure 1, flies are presented with one odor (apple or orange) and the aversive flavor (apple or orange juice supplemented with quinine in the learning assay, without quinine in the olfactory preference assay). (B) In Exposure 2, flies are presented the second odor (orange or apple) and the second flavor (orange or apple juice without quinine). (C) At Test, both stimuli (orange and apple odor) are presented (without quinine) on different sides of the apparatus.
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fig-1: T-maze apparatus and experimental paradigm.(A) During Exposure 1, flies are presented with one odor (apple or orange) and the aversive flavor (apple or orange juice supplemented with quinine in the learning assay, without quinine in the olfactory preference assay). (B) In Exposure 2, flies are presented the second odor (orange or apple) and the second flavor (orange or apple juice without quinine). (C) At Test, both stimuli (orange and apple odor) are presented (without quinine) on different sides of the apparatus.

Mentions: The T-maze (31 × 17.5 cm) used for the experimental assays (Fig. 1A) consisted of a starting chamber and a central chamber (12 × 8 × 1.5 cm) connected on each side to a food chamber. The starting chamber (9.5 × 2.5 cm) contained the flies at the beginning of each experimental phase. Food chambers (9.5 × 2.5 cm) were filled with 4 ml of experimental food. In each experimental phase, flies begun the exploration of the apparatus from the starting chamber. The central chamber was connected to the food chambers with a funnel that prevents flies to re-enter the central chamber once they have approached the food. A similar trapping technique has been previously used for fruit flies.


Large-scale assessment of olfactory preferences and learning in Drosophila melanogaster: behavioral and genetic components.

Versace E, Reisenberger J - PeerJ (2015)

T-maze apparatus and experimental paradigm.(A) During Exposure 1, flies are presented with one odor (apple or orange) and the aversive flavor (apple or orange juice supplemented with quinine in the learning assay, without quinine in the olfactory preference assay). (B) In Exposure 2, flies are presented the second odor (orange or apple) and the second flavor (orange or apple juice without quinine). (C) At Test, both stimuli (orange and apple odor) are presented (without quinine) on different sides of the apparatus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562235&req=5

fig-1: T-maze apparatus and experimental paradigm.(A) During Exposure 1, flies are presented with one odor (apple or orange) and the aversive flavor (apple or orange juice supplemented with quinine in the learning assay, without quinine in the olfactory preference assay). (B) In Exposure 2, flies are presented the second odor (orange or apple) and the second flavor (orange or apple juice without quinine). (C) At Test, both stimuli (orange and apple odor) are presented (without quinine) on different sides of the apparatus.
Mentions: The T-maze (31 × 17.5 cm) used for the experimental assays (Fig. 1A) consisted of a starting chamber and a central chamber (12 × 8 × 1.5 cm) connected on each side to a food chamber. The starting chamber (9.5 × 2.5 cm) contained the flies at the beginning of each experimental phase. Food chambers (9.5 × 2.5 cm) were filled with 4 ml of experimental food. In each experimental phase, flies begun the exploration of the apparatus from the starting chamber. The central chamber was connected to the food chambers with a funnel that prevents flies to re-enter the central chamber once they have approached the food. A similar trapping technique has been previously used for fruit flies.

Bottom Line: Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples.Consistent with previous results, we show that flies have a preference for orange vs. apple odor.We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut für Populationsgenetik , Vetmeduni, Vienna , Austria ; Center for Mind/Brain Sciences, University of Trento , Rovereto , Italy.

ABSTRACT
In the Evolve and Resequence method (E&R), experimental evolution and genomics are combined to investigate evolutionary dynamics and the genotype-phenotype link. As other genomic approaches, this methods requires many replicates with large population sizes, which imposes severe restrictions on the analysis of behavioral phenotypes. Aiming to use E&R for investigating the evolution of behavior in Drosophila, we have developed a simple and effective method to assess spontaneous olfactory preferences and learning in large samples of fruit flies using a T-maze. We tested this procedure on (a) a large wild-caught population and (b) 11 isofemale lines of Drosophila melanogaster. Compared to previous methods, this procedure reduces the environmental noise and allows for the analysis of large population samples. Consistent with previous results, we show that flies have a preference for orange vs. apple odor. With our procedure wild-derived flies exhibit olfactory learning in the absence of previous laboratory selection. Furthermore, we find genetic differences in the olfactory learning with relatively high heritability. We propose this large-scale method as an effective tool for E&R and genome-wide association studies on olfactory preferences and learning.

No MeSH data available.


Related in: MedlinePlus