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Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus

Representative Western blots showing the effect of oxygen tension, pH, and IL-1β on protein expression of superoxide dismutase 1 (Cu/ZnSOD, SOD1), superoxide dismutase 2 (MnSOD, SOD2), and α-tubulin from equine articular chondrocytes. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 or pH6.2 in the presence or absence of 10 ng/ml IL-1β (A), in the presence of resveratrol (10 µM) (B) or N-acetylcysteine (2 mM) (C).
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f0025: Representative Western blots showing the effect of oxygen tension, pH, and IL-1β on protein expression of superoxide dismutase 1 (Cu/ZnSOD, SOD1), superoxide dismutase 2 (MnSOD, SOD2), and α-tubulin from equine articular chondrocytes. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 or pH6.2 in the presence or absence of 10 ng/ml IL-1β (A), in the presence of resveratrol (10 µM) (B) or N-acetylcysteine (2 mM) (C).

Mentions: Expression of SOD1 was significantly decreased under very low oxygen conditions (<1% O2) at both pH 7.2 and 6.2 (P=0.03 and 0.01, respectively) and in the presence of IL-1β (P=0.002 and 0.002, respectively) (Fig. 5A and Fig. S4A–D).IL-1β also significantly reduced SOD2 protein expression under very low oxygen conditions at pH 7.2 and 6.2 (P=0.036 and 0.024, respectively) (Fig. 5A and Fig. S5C and D).Both resveratrol and NAC restored SOD1 and 2 protein expression under very low oxygen conditions (<1% O2) and in the presence of IL-1β (Fig. 5B and C).


Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Representative Western blots showing the effect of oxygen tension, pH, and IL-1β on protein expression of superoxide dismutase 1 (Cu/ZnSOD, SOD1), superoxide dismutase 2 (MnSOD, SOD2), and α-tubulin from equine articular chondrocytes. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 or pH6.2 in the presence or absence of 10 ng/ml IL-1β (A), in the presence of resveratrol (10 µM) (B) or N-acetylcysteine (2 mM) (C).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562226&req=5

f0025: Representative Western blots showing the effect of oxygen tension, pH, and IL-1β on protein expression of superoxide dismutase 1 (Cu/ZnSOD, SOD1), superoxide dismutase 2 (MnSOD, SOD2), and α-tubulin from equine articular chondrocytes. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 or pH6.2 in the presence or absence of 10 ng/ml IL-1β (A), in the presence of resveratrol (10 µM) (B) or N-acetylcysteine (2 mM) (C).
Mentions: Expression of SOD1 was significantly decreased under very low oxygen conditions (<1% O2) at both pH 7.2 and 6.2 (P=0.03 and 0.01, respectively) and in the presence of IL-1β (P=0.002 and 0.002, respectively) (Fig. 5A and Fig. S4A–D).IL-1β also significantly reduced SOD2 protein expression under very low oxygen conditions at pH 7.2 and 6.2 (P=0.036 and 0.024, respectively) (Fig. 5A and Fig. S5C and D).Both resveratrol and NAC restored SOD1 and 2 protein expression under very low oxygen conditions (<1% O2) and in the presence of IL-1β (Fig. 5B and C).

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus