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Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus

Effect of oxygen tension, pH, and IL-1β on GSH:GSSG ratio in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3Dalginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GSH:GSSG ratio was calculated using the GSH/GSSG-Glo assay. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
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f0020: Effect of oxygen tension, pH, and IL-1β on GSH:GSSG ratio in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3Dalginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GSH:GSSG ratio was calculated using the GSH/GSSG-Glo assay. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.

Mentions: The ratio of reduced (GSH) to oxidized glutathione (GSSG) is an indicator of cellular redox status. GSH:GSSG ratio was significantly reduced under acidic conditions under very low oxygen conditions (<1% O2, pH 6.2) (P=0.004) as well as under acidic conditions at 21% O2(P=0.004) (Fig. 4A and B). At 2 and 5% O2 (pH 6.2), GSH:GSSG ratio was reduced but this was not significant. IL-1β markedly reduced GSH:GSSG ratio under all conditions (Fig. 4C and D). Interestingly NACsignificantly increased GSH:GSSG ratio above control levels, particularly in pH 7.2 and negated the effects of acidosis and low oxygen particularly in the presence of IL-1β. Resveratrol had little effect on GSH:GSSG ratio under acidic or low oxygen conditions in the presence or absence of IL-1β. Changes in GSH:GSSG ratio were primarily due to alterations in cellular GSH levels (Fig. S3).


Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Effect of oxygen tension, pH, and IL-1β on GSH:GSSG ratio in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3Dalginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GSH:GSSG ratio was calculated using the GSH/GSSG-Glo assay. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4562226&req=5

f0020: Effect of oxygen tension, pH, and IL-1β on GSH:GSSG ratio in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3Dalginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GSH:GSSG ratio was calculated using the GSH/GSSG-Glo assay. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
Mentions: The ratio of reduced (GSH) to oxidized glutathione (GSSG) is an indicator of cellular redox status. GSH:GSSG ratio was significantly reduced under acidic conditions under very low oxygen conditions (<1% O2, pH 6.2) (P=0.004) as well as under acidic conditions at 21% O2(P=0.004) (Fig. 4A and B). At 2 and 5% O2 (pH 6.2), GSH:GSSG ratio was reduced but this was not significant. IL-1β markedly reduced GSH:GSSG ratio under all conditions (Fig. 4C and D). Interestingly NACsignificantly increased GSH:GSSG ratio above control levels, particularly in pH 7.2 and negated the effects of acidosis and low oxygen particularly in the presence of IL-1β. Resveratrol had little effect on GSH:GSSG ratio under acidic or low oxygen conditions in the presence or absence of IL-1β. Changes in GSH:GSSG ratio were primarily due to alterations in cellular GSH levels (Fig. S3).

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus